Journal of Food, Agriculture and Environment

Vol 6, Issue 2,2008
Online ISSN: 1459-0263
Print ISSN: 1459-0255

Transient expression of β-glucuronidase reporter gene in date palm (Phoenix dactylifera L.) embryogenic calli and somatic embryos via microprojectile bombardment


Ali A. Habashi 1*, Mina Kaviani 1, Amir Mousavi 2 , Soghra Khoshkam 1

Recieved Date: 2007-09-08, Accepted Date: 2008-03-28


The date palm, Phoenix dactylifera L. ‘Khorma’ (Persian), is an example of a tree crop that can benefit immediately from the applications of the modern biotechnology. Despite the importance as an economic plant, there is no report on its genetic transformation. Palm tree is the target for several pests and diseases, so it is necessary to focus on its in vitro propagation and genetic engineering technology (date palm transformation) to overcome some of these problems. The objective of this study was to identify the major parameters controlling DNA delivery by particle bombardment to date palm embryogenic calli and somatic embryos. The main factors studied were osmotic conditioning of explants before and after bombardment (osmotic and non-osmotic), type of explants (embryogenic callus and somatic embryo) and different bombardment parameters like acceleration pressure (900 and 1100 psi), bombardment distance (6 and 9 cm) and gold particle size (1 and 0.6 µm). Efficiency of DNA (gus gene) delivery was assessed by scoring transient GUS expression in bombarded tissues. Statistical analysis of blue spots produced by GUS histochemical assay showed that all parameters significantly affected the transient GUS expression. Results of mean comparison showed that GUS transient expression increased when 1.0 µm gold particle, 1100-psi rupture disk, 6 cm bombardment distance, osmotic medium and mature somatic embryos were used.


Particle bombardment, DNA delivery, histochemical assay

Journal: Journal of Food, Agriculture and Environment
Year: 2008
Volume: 6
Issue: 2
Category: Agriculture
Pages: 160-163

Full text for Subscribers

Note to users

The requested document is freely available only to subscribers/registered users with an online subscription to the Journal of Food, Agriculture & Environment. If you have set up a personal subscription to this title please enter your user name and password. All abstracts are available for free.

Article purchasing

If you like to purchase this specific document such as article, review or this journal issue, contact us. Specify the title of the article or review, issue, number, volume and date of the publication. Software and compilation, Science & Technology, all rights reserved. Your use of this website details or service is governed by terms of use. Authors are invited to check from time to time news or information.

Purchase this Article:   20 Purchase PDF Order Reprints for 15

Share this article :