Journal of Food, Agriculture and Environment

Vol 9, Issue 1,2011
Online ISSN: 1459-0263
Print ISSN: 1459-0255

ITS-nrDNA sequence study of important species in genus Cytospora


Zhong-dong Yu 1*, E-jiao Ma 2, JunYang 2

Recieved Date: 2010-10-29, Accepted Date: 2011-01-10


ITS sequences of Cytospora, combined the related sequences in GenBank, were analyzed by Neighbor Joint Method of Software Mega 4.0. The results show that all tested Cytospora isolates can be divided into two groups, and five subgroups in Group1. Group 2 is a mixture group of poplar rot pathogens and pear rot pathogens. The first subgroup in Group1 is eventually poplar Cytospora pathogens. The second subgroup is made of pathogens of apple rot pathogen and pear rot pathogen. The third subgroup is Cytospora cincta. The fourth subgroup is Lecucotosma persooni, while the fifth subgroup is the rot pathogens all from host Populus cathayana. The overall diversity of Cytospora sequences is 0.1789, Tajima-Nei value is 0.254762, which manifests Cytospora is a species deviated from Hardy-Weinberg Balance, and positive mutant events in the progress of evolution are distinguished. The effects of environment factors to the evolution progress are dominated. Poplar Cytospora pathogens from China are subjected to 3 clusters, total diversity of intra-species sequences is 0.180102, Tajima-Nei value is 0.139, which also imply the positive mutant events in the evolution progress. Most isolates of Cytospora chrysosperma are subject to Subgroup 1, and coincided with the sexual isolates of Valsa sordida from GenBank, which verified the consistence between asexual Cytospora chrysosperma and sexual Valsa sordida. On the other hand, the phylogeny tree also shows Cytospora chrsosperma isolates in China are more homogenous with isolates in America and Iran than those in UK. The relationships between C. chrysosperma and C. mandshuriaC. ambiens and C. mandshuria are not clear in this paper, and ITS sequences are not enough to distinguish them if there is no other gene’s assistance.


Cytospora, C. chrysosperma (Pers.) Fr., ITS, diversity, phylogeny

Journal: Journal of Food, Agriculture and Environment
Year: 2011
Volume: 9
Issue: 1
Category: Environment
Pages: 557-562

Full text for Subscribers

Note to users

The requested document is freely available only to subscribers/registered users with an online subscription to the Journal of Food, Agriculture & Environment. If you have set up a personal subscription to this title please enter your user name and password. All abstracts are available for free.

Article purchasing

If you like to purchase this specific document such as article, review or this journal issue, contact us. Specify the title of the article or review, issue, number, volume and date of the publication. Software and compilation, Science & Technology, all rights reserved. Your use of this website details or service is governed by terms of use. Authors are invited to check from time to time news or information.

Purchase this Article:   20 Purchase PDF Order Reprints for 15

Share this article :