Journal of Food, Agriculture and Environment

Production medium optimization for enhancement the exotoxin secretion by Pseudomonas aeruginosa

Author(s):

Mary S. Khalil ¹, Tarek A. A. Moussa ¹*, Tahany M. A. Abdel-Rahman ¹, Baydaa H. Alwan ^{1, 2}

Recieved Date: 2013-06-09, Accepted Date: 2013-10-24

Abstract:

Media variations including eight variables were optimized for improvement of exotoxin production by Pseudomonas aeruginosa, using the statistical design Taguchi system. Eighteen experimental trials with final layout of L 18 (1² x 7³) were used. The bacterial toxin production varied significantly among media within range of 1.0-31.0 µg ml^-1. This indicated the validity of optimization design in bioprocesses. The statistical design showed little variations between the actual experimental values and the Taguchi software predicted values. The (ANOVA) analysis implies “P-value” of 10.4 which revealed that the design model is significant. The F-value (less than 0.05) revealed that temperature, agitation and interaction between incubation period and beef extract are the most significant model terms. The regression values of the classical sum of squares indicated that predicted-R² (0.6208) and the adjusted-R² (0.8341) were in reasonable agreement which indicates the accuracy of experimentation. The predicted optimum medium from the Taguchi software database was beef extract 4 g l^-1, NaCl 7 g l^-1 and peptone 3 g l^-1, incubation period 36 h, pH 6.5, temperature 27^oC, inoculum size 3% and agitation 150 rpm.

Keywords:

Pseudomonas aeruginosa, exotoxins, statistical design, production medium

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Journal: Journal of Food, Agriculture and Environment
Year: 2013
Volume: 11
Issue: 3&4
Category: Food and Health
Pages: 173-176

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