Journal of Food, Agriculture and Environment

Influence of the auxin-like activity of humic acid on bio and microbiometric parameters of Pisum sativum L. by in vitro cultures of pea plants 

Author(s):

Andrzej Gawlik ¹*, Danuta Kulpa ², Dorota Gołębiowska ¹, Romualda Bejger ¹

Recieved Date: 2014-03-10, Accepted Date: 2014-09-20

Abstract:

Humic acids (HA) can be found in all aquatic and land-based ecosystems. They may directly or indirectly influence the growth of plants. The aim of the study was to determine the influence of HA extracted from peloid on the growth and morphological features of pea plants by in vitro cultures. Sterilized pea seeds (Pisum sativum L.) of Ramdrod variety were placed on Murashige & Skoog (MS) medium with HA of the following concentration, expressed as content of HA carbon in dm³ of medium: 0.005; 0.01 and 0.02 (gC_HA·dm^-3). Control samples included those in which HA was not added to MS medium. After two weeks of the experiment, morphological features were determined in young pea plants and microbiometric features examined using the scanning electron microscope (SEM). Biometric measurements showed that HA added to the medium caused statistically significant increase in the height of the pea plant shoot, as well as longer roots in comparison with control samples. Additionally, growth of fresh weight of the shoot was observed, whereas in the case of the highest and the lowest concentration of HA a significant increase in fresh and dry weight of roots. Examination using the SEM showed that humic acids had positive influence on the length of internodal shoot and root cells. At the same time, a decrease in the number of stomas was observed. The experiment showed that HA activity is similar to auxins. No influence of HA on the green index and dry weight of shoots were recorded.

Keywords:

Auxin-like activity of humic acid, biometric and microbiometric parameters of pea plants, Pisum sativum L., scanning electron microscope

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Journal: Journal of Food, Agriculture and Environment
Year: 2014
Volume: 12
Issue: 3&4
Category: Agriculture
Pages: 209-212

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