Journal of Food, Agriculture and Environment
Vol 7,
Issue 2,2009
Online ISSN: 1459-0263
Print ISSN: 1459-0255
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In vivo antioxidative capacities of rapeseed meal polysaccharides
Author(s):
Hanju Sun *,
Shaotong Jiang, Pan Mu, Ding QiRecieved Date: 2009-01-08, Accepted Date: 2009-03-19
Abstract:In the current research, rapeseed meal was first defatted with petroleum ether and removed coloured and low molecular weight compounds with 95% (v/v) ethanol in turn. It was then extracted with 9 vol of 0.3% NaOH aqueous solution, followed by several purification steps. The resulted polysaccharides were daily administered to mice via a gastric gavage at doses of 50, 100 and 200 mgkg-1 BW, respectively, for 30 days. Vitamin C and α-tocopherol were used as positive controls. After the experimental period, the in vivo antioxidative capacities of PRM were evaluated using different assays, including glutathione peroxidase (GSH-Px), catalase (CAT) and superoxide dismutase (SOD), total antioxidant capacity (T-AOC) and malondialdehyde (MDA). These antioxidant indexes were also compared with those of standard antioxidants (vitamin C and α-tocopherol). The results indicated that PRM markedly increased the activities of GSH-Px, CAT and SOD and T-AOC levels and decreased MDA levels. The findings suggest that PRM have strong in vivo antioxidant capacities and may be recommended as health food in the future.
Keywords:Rapeseed meal,
polysaccharide, mice, in vivo, antioxidative capacityJournal: Journal of Food, Agriculture and Environment
Year: 2009
Volume: 7
Issue: 2
Category: Food and Health
Pages: 97-102
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