Journal of Food, Agriculture and Environment

Breeding of L-arginine-producing strain and optimization of fermentation conditions

Author(s):

Yulong Yin ^1*, Xiangshan Tang ^{1, 2}, Zhou Dong ^{1, 2}, Zhi-Ru Tang ³

Recieved Date: 2011-07-03, Accepted Date: 2011-09-28

Abstract:

A L-arginine-producing mutant Corynebacterium glutamicum ZD2009 was selected out by arginine-methylester (AE) resistance after stepwise mutagenic treatment with N-methyl-N-nitro-N-nitrosoguanidine (NTG) from Corynebacterium glutamicum DSM1412. This mutant also has good stability of descendibility of L-arginine. After investigating fermentation medium by orthogonal analysis, results showed optimal medium containing 2 glucose 9.0%, corn steep liquor 3.0%, (NH₄)₂SO₄ 6.0%, KH₂PO₄ 0.05%, MgSO₄·7H₂O 0.03% and calcium carbonate 2.0%, inoculum size 6%, pH 7.0, liquid volume 15 ml/100 ml. Mutant Corynebacterium glutamicum ZD2009 could accumulate L-arginine 44 g/l, on average, which increased about 41.9% more than before in this optimized medium when it was cultured at 30°C for 4 days.

Keywords:

L-arginine, Corynebacterium glutamicum, fermentation, mutation breeding

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Journal: Journal of Food, Agriculture and Environment
Year: 2011
Volume: 9
Issue: 3&4
Category: Agriculture
Pages: 608-612

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