Journal of Food, Agriculture and Environment

Genetic diversity of pea (Pisum sativum L.) genotypes assessed by pedigree, morphological and molecular data

Author(s):

Tihomir Cupic ^1*, Marijana Tucak ¹, Svetislav Popovic ¹, Snjezana Bolaric ², Sonja Grljusic ¹, Vinko Kozumplik ²

Recieved Date: 2009-07-15, Accepted Date: 2009-10-02

Abstract:

A considerable loss of pea landraces and consequential loss of genetic variability in European countries and other areas has been recorded. Evolutionary and historical information as well as information about extent of various gene pools uses are extremely valuable when planning which of germplasm to use in germplasm-enhancement or base broadening initiatives. Simple sequence repeat (SSR) markers, morphological traits and pedigree data were used to analyse genetic diversity of European pea (Pisum sativum L.) germplasm, to determine differences between P. sativum var. arvense and P. sativum ssp. sativum groups, and to estimate genetic variability among and within eighteen P. sativum accessions. Coancestry coefficients across investigated accessions varied from 0.46 to 1.00. The average dissimilarity index between Pisum sativum var. arvense and P. sativum ssp. sativum groups was 0.99, where estimates obtained by pedigree data might be overestimated. Average morphological distance among all accessions was slightly higher than average molecular distance (0.62 and 0.59, respectively). Average morphological distance between P. sativum ssp. sativum and P. sativum var. arvense groups was also higher than average molecular distance (0.71 and 0.69, respectively). Results, according to morphological traits used in this study were well suited to assess differences among accessions. Accessions were grouped according to their botanical characters and agronomic use. Genetic distances estimated by molecular marker (SSR) data in comparison with distances estimated by conventional methods (pedigree and morphologic traits) showed higher similarity with genetic distances estimated by morphological data. Results indicated that intercrosses between arvense and sativum accessions as well as inclusion of valuable landraces into breeding programmes might prevent loss of diversity in the Pisum gene pool.

Keywords:

Pisum sativum L., interspecies relationships, diversity, genetic variability, pedigree data, morphological data, molecular data

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Journal: Journal of Food, Agriculture and Environment
Year: 2009
Volume: 7
Issue: 3&4
Category: Agriculture
Pages: 343-348

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