Journal of Food, Agriculture and Environment

Molecular characterization of Salmonella virulence genes isolated from different sources relevant to human health 

Author(s):

Ihab Mohamed Moussa ¹,   Yosra  Samy Aleslamboly  ², Abdullah Abdulaziz Al-Arfaj ¹, Ashgan Mohamed Hessain ³*, Azza Said Gouda ⁴, Refai Mohamed Kamal ²

Recieved Date: 2013-01-10, Accepted Date: 2013-04-26

Abstract:

Between 14 January 2010 to 30 April 2012, bacteriological examination of 1075 samples (290 rectal swabs from cattle, 335 rectal swabs from sheep, 450 cloacal swabs from poultry) from diarrheic animals revealed the isolation of 68 Salmonella belonging to 13 different Salmonella serovars. This work was done at Faculty of Veterinary Medicine, Cairo University as well as Center of Excellence in Biotechnology Research, King Saud University. The most common serovars were Salmonella Typhimurium (16 isolates), Salmonella Enteritidis (13 isolates), Salmonella Kentucky (8 isolates) and Salmonella Arizona (7 isolates). Other serovars typed were Salmonella Heidelberg (4), Salmonella Cerro (4), Salmonella Gallinarum (3), Salmonella Virginia (3), Salmonella Paratyphi-A (3), Salmonella Dublin (2), Salmonella Agona (2), Salmonella Hadar (2) and Salmonella Bardo (1). All isolates of Salmonella Enteritidis and Salmonella Typhimurium were examined for the virulence genes fimA, vag, and invA and spvCgenes by Multiplex PCR. It was evident that fimA was the most common in both serovars, followed by invA, while vag and spvC had almost equal prevalence Salmonella Enteritidis and Salmonella Typhimurium isolates from cattle, sheep, poultry and human. 

Keywords:

Salmonella Typhimurium, Salmonella Enteritidis, virulence genes fimA, vag, invA, spvCgenes, m-PCR

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Journal: Journal of Food, Agriculture and Environment
Year: 2013
Volume: 11
Issue: 2
Category: Food and Health
Pages: 197-201

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