Journal of Food, Agriculture and Environment
Vol 20,
Issue 3&4,2022
Online ISSN: 1459-0263
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Phytochemical, antimicrobial and antioxidant assessment of leafy vegetables collected from solid waste dumping ground and normal ground - A comparative study
Author(s):
Paramita Barman,
Debasree Ghosh, Ayan SahaRecieved Date: 2022-08-11, Accepted Date: 2022-09-06
Abstract:Vegetables having antimicrobial activities play significant role against some pathogenic microorganisms. This study aims to compare moisture content, antioxidant content, antimicrobial activity and presence of plant derived secondary metabolites namely phytochemicals of some selected and locally available vegetables collected from solid waste dumping ground (SWDG) at Titagarh, Kolkata, West Bengal, India and from normal ground (NG) namely bottle gourd (Lagenaria siceraria), red amaranth (Amaranthus cruentus) and Ceylon spinach (Basella alba). Matured, disease free and fresh leafy samples were collected from different localities. Vegetables collected from NG showed significantly higher moisture content compared to SWDG, which was 82.91+2.44 followed by 73.88+1.62 and 71.72+2.58 from leaves of L. siceraria, A. cruentus and B. alba. Qualitative analysis of phytochemicals of methanol extracted vegetables collected from SWDG showed greater phytochemical properties than from NG. In case of comparing antimicrobial activity, the bacterial cell suspensions were adjusted to turbidity standard (10-5) at approximately 3.2× 107 CFU. Disc diffusion method was applied for antimicrobial assessment at different concentrations (50 μl and 100 μl) against Gram negative organisms. The inhibitory action was indicated in almost all raw vegetables but, vegetables having concentrations of 50 μl and 100 μl, showed no inhibitory effect on the tested organism except leaves of L. siceraria (100 μl). Among these vegetables collected from SWDG, showed maximum inhibitory effect in leaves of L. siceraria of 20.33+ 1.52 mm followed by B. alba of 17.33 mm and in A. cruentus of 19.33 mm against Gram negative organism. As no such specific microbial strain was used for this test, after the antimicrobial analysis, we assumed by comparing the standard value given by the clinical and laboratory standards institute (CLSI) it might be belong to the Enterobacteriaceae (Gram negative) family. In this study we can conclude that, higher the amount of phytochemicals present in vegetable leaves, the higher would be the antimicrobial property.
Keywords:Antioxidant activity,
phytochemical screening, disc diffusion method, antimicrobial activity, antibacterial susceptibility test, SWDG, NG, CFUJournal: Journal of Food, Agriculture and Environment
Year: 2022
Volume: 20
Issue: 3&4
Category: Environment
Pages: 90-95
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