Journal of Food, Agriculture and Environment

Bacterial biofertilizers: An innovative green biotechnological approach for sustainable growth and biomass accumulation in Eucalyptus clones


Gulab Pandove 1*, Madhurama Gangwar 2, Avtar Singh 1

Recieved Date: 2016-09-16, Accepted Date: 2016-12-22


A field experiment was carried out to study the effect of bacterial biofertilizer/green biotechnology on vegetative growth parameters and biomass accumulation of Eucalyptus clones under field conditions. For the preparation of bacterial biofertilizer [Charcoal based carrier, 1X108 colony forming unit (CFU) per gram of carrier] standard culture of Azotobacter sp. was obtained from the Department of Microbiology, Punjab Agricultural University, Ludhiana, Punjab, India and another potential culture of biofertilizer (ND-PAU2-YELLOW) was isolated from degraded paddy straw. This culture was characterized at molecular level by sequencing of 16SrDNA as Sphingobacterium sp. Both the culture used in this study were characterized for their plant growth promoting traits and used @100 gm/plant respectively as basal dose along with farm yard manure (2.0 kg/plant) at the time of time of transplanting under field conditions.Results of experiment showed that three month old Eucalyptus clones transplanted under field conditions showed very good response to Azotobacter sp. and Sphingobacterium sp. treatment (T3) followed by Azotobacter sp. (T2). After one eyar, the data revealed that dual inoculation of Azotobacter sp. and Sphingobacterium sp. produced 13.58 per cent increase in height (301.00 cm), 14.05 per cent in collar diameter (4.14 cm), 15.90 per cent in root length (52.70), 19.09 per cent no. of branches/plant (43.67), 30.30 per cent in fresh weight of shoot (2.58 kg), 27.86 per cent in dry weight of shoot (1.56 kg), 20.00 per cent in fresh weight of root (0.54 kg) and 26.08 percent in dry weight of root (0.29 kg). Thus dual inoculation of biofertilizers could play an important role in the establishment of agroforestry tree species under field condition by increasing the availability of nutrients by biological nitrogen fixation and phosphate solubilisation in addition to providing biologically active substances like IAA.


Eucalyptus clones, IAA production, phosphate solubilisation activity, Azotobacter sp., Sphingobacterium sp.

Journal: Journal of Food, Agriculture and Environment
Year: 2017
Volume: 15
Issue: 1
Category: Environment
Pages: 56-60

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