Journal of Food, Agriculture and Environment

Vol 7, Issue 1,2009
Online ISSN: 1459-0263
Print ISSN: 1459-0255

Assessment of genetic diversity in Trigonella foenum graecum Tunisian cultivars using ISSR markers


Nidhal Marzougui 1*, Anissa Boubaya 1, Walid Elfalleh 1, Ferdaous Guasmi 1, Leila Laaraiedh 1, Ali Ferchichi 1, Tebra Triki 1, Mohamed Beji 2

Recieved Date: 2008-10-08, Accepted Date: 2008-12-11


Trigonella foenum graecum is an annual flowering species largely cultivated in the Mediterranean basin countries, central Asia, central Europe, United Kingdom and United States. The grain is rarely grown outside their native habitat. This work aims to study the diversity of 38 Tunisian cultivars of this plant using Inter Simple Sequence Repeat markers. Cultivars were collected from upper-semi arid, sub humid and lower arid Tunisian bioclimatic stages. A set of 12 ISSR primers was used for cultivars initial screening. Four primers gave amplification, detected intraspecific variation and revealed 144 bands. Sixty-two bands were polymorphic with an average of 15.5 bands per primer and 60.5% of polymorphism. The percentage of polymorphic bands varied from 30.7% for (CA)GT primer to 73.3% for (GAG)3GC primer. The highest heterozygosity value (0.95) was obtained with (GA)6CC primer and the lowest one (0.5) was observed with (CA)6GT primer. The highest marker index value (3.6) was recorded with (GAG)3GC primer and the lowest one (2.25) was noted with (CA)6AG. UPGMA dendrogram presented five cultivar clusters. Principal components analysis confirmed this clustering and showed the same five clusters that were obtained by UPGMA analysis. Genetic distances were not correlated with geographic distances (r = 0.04 and p = 0.366>0.05) but the majority of the cultivars were localized in the upper semi arid stage. This study confirmed the hypothesis of the Near East and Mediterranean Centers to be the centers of origin and/or diversity of Trigonella and suggested that Tunisia should be added to the list of countries belonging to the Mediterranean Center.


Trigonella foenum graecum, genetic diversity, bioclimatic stage, Tunisia, ISSR, polymorphism, heterozygosity, marker index, UPGMA, principal components analysis, center of origin and/or diversity

Journal: Journal of Food, Agriculture and Environment
Year: 2009
Volume: 7
Issue: 1
Category: Agriculture
Pages: 101-105

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