Journal of Food, Agriculture and Environment
Vol 8,
Issue 1,2010
Online ISSN: 1459-0263
Print ISSN: 1459-0255
|
|
|
|
Newsletter
Subscribe to our newsletter and stay up to date with the latest publishes
SSR marker-based mapping and linkage analysis of Bombyx mori thermotolerance gene
Author(s):
Yuan Zhao 1, 2*,
Jiang Zhang 2, Yang-Chun Wu 1, Ya-Hong Zhu 1Recieved Date: 2009-08-29, Accepted Date: 2009-12-28
Abstract:Based on the characteristic that there is crossing-over in male Bombyx mori but no crossing-over in female Bombyx mori, Dong34, a thermotolerant strain, and Ou17, a highly susceptible strain, were used to produce two backcross populations, namely, (Ou17 X Dong34)♀ X Ou17♂ and Ou17♀ X (Ou17 X Dong34)♂, which were designated as BC1F and BC1M, respectively. With 84 specific primers for molecular markers selected from published SSR marker-based molecular linkage map, twenty two BC1F individuals were used to screen simple sequence repeat (SSR) molecular markers linked to thermotolerance gene (KN), and 5 SSR molecular markers on the 8th linkage group were found to link to KN. Subsequently, 192 BC1M individuals were used to map these markers and KN, and a linkage map was constructed, where the order of the five SSR molecular markers and KN are LFL1123, LFL0407, KN, LFL0329, LFL0658 and LFL0944.
Keywords:Bombyx mori,
SSR molecular marker, linkage, mappingJournal: Journal of Food, Agriculture and Environment
Year: 2010
Volume: 8
Issue: 1
Category: Environment
Pages: 338-342
Full text for Subscribers
Information:
Note to users
The requested document is freely available only to subscribers/registered users with an online subscription to the Journal of Food, Agriculture & Environment. If you have set up a personal subscription to this title please enter your user name and password. All abstracts are available for free.
Article purchasing
If you like to purchase this specific document such as article, review or this journal issue, contact us. Specify the title of the article or review, issue, number, volume and date of the publication. Software and compilation, Science & Technology, all rights reserved. Your use of this website details or service is governed by terms of use. Authors are invited to check from time to time news or information.
Purchase this Article: 20 Purchase PDF Order Reprints for 15
Share this article :