Journal of Food, Agriculture and Environment

Vol 8, Issue 2,2010
Online ISSN: 1459-0263
Print ISSN: 1459-0255

Evaluation of Salmonella and Listeria monocytogenes contamination on leafy green vegetables


Sait Aykut Aytac 1*, Ulya Ben 1, Canan Cengiz 1, Birce Mercanoglu Taban 2

Recieved Date: 2010-01-15, Accepted Date: 2010-04-22


The objective of this study was to monitor Salmonella and Listeria monocytogenes contamination of leafy green vegetables produced in urban and periurban agricultural fields with natural water irrigation. A total of 164 samples comprising of different leafy green vegetables (8 basils, 15 dills, 20 garden cresses, 16 kales, 12 lettuces, 19 mints, 19 parsleys, 18 purslanes, 1 radish, 20 rockets, 14 scallions and 2 spinaches) were randomly collected from nearby agricultural fields in the close vicinity of Ankara Stream over an 8-month period between April 2007 and November 2007. Samples were examined for Salmonella and L. monocytogenes using ISO methods with immunomagnetic separation (IMS) and then polymerase chain reaction (PCR). No Salmonella and L. monocytogenes was detected from the 25-g radish, spinach and scallion samples tested. However, 23 samples (1 basil, 2 dills, 1 garden cress, 1 kale, 2 lettuces, 4 mints, 3 parsleys, 5 purslanes and 4 rockets) were positive for Salmonella and 14 samples (3 basils, 1 dill, 1 garden cress, 2 kales, 1 lettuce, 1 mint, 2 parsleys, 1 purslane and 2 rockets) were positive for L. monocytogenes. Overall, important contamination of leafy green vegetables by Salmonella and L. monocytogenes was observed. Therefore, this study will draw attention to the production of freshly consumed leafy green vegetables and so will aid in the development of control measures for these pathogens before harvesting and will highlight the importance of their intake which may cause a communicable disease and so pose a serious threat to both environment and human health.


Contamination, detection, immunomagnetic separation (IMS), leafy green vegetable, Listeria monocytogenes, polymerase chain reaction (PCR), Salmonella

Journal: Journal of Food, Agriculture and Environment
Year: 2010
Volume: 8
Issue: 2
Category: Food and Health
Pages: 275-279

Full text for Subscribers

Note to users

The requested document is freely available only to subscribers/registered users with an online subscription to the Journal of Food, Agriculture & Environment. If you have set up a personal subscription to this title please enter your user name and password. All abstracts are available for free.

Article purchasing

If you like to purchase this specific document such as article, review or this journal issue, contact us. Specify the title of the article or review, issue, number, volume and date of the publication. Software and compilation, Science & Technology, all rights reserved. Your use of this website details or service is governed by terms of use. Authors are invited to check from time to time news or information.

Purchase this Article:   20 Purchase PDF Order Reprints for 15

Share this article :