Journal of Food, Agriculture and Environment

Effect of water stress on lipid peroxidation and antioxidant enzymes in local bread wheat hexaploids

Author(s):

Nadia Khan, Farzana Nasir Naqvi ^*

Recieved Date: 2009-11-30, Accepted Date: 2010-03-22

Abstract:

Current research was conducted with the objective to observe the changes in activities of antioxidant enzymes and malondialdehyde (MDA) content which may play a part in distinguishing tolerant and susceptible genotypes under different irrigation levels. The experimental material comprised of germplasm of eight local bread wheat hexaploids. Stress was imposed by withholding irrigation at three different growth stages of plant, i.e. vegetative, anthesis and vegetative + anthesis. Experiment was carried out in randomized complete block design in a split-plot arrangement. Water regimes (irrigated and non-irrigated) were allocated to the main plots and genotypes to the sub-plots. Second leaves were collected at respective stages of stresses (non-irrigated) along with control samples (irrigated). A variable response of genotypes was observed at different water regimes. Elevated MDA levels at vegetative stage indicated more sensitivity at young plants to oxidative stress than at mature plants. The response of peroxidase and superoxide dismutase to stress imposed at vegetative stage was towards promotion. As the water stress moved towards anthesis, an inhibition was noted. Enhancement was again observed at vegetative + anthesis stress in the activity of peroxidase whereas superoxide dismutase declined at anthesis and vegetative + anthesis stress. When stress was imposed at three levels of irrigation, minimum lipid peroxidation with high peroxidase activity has been found in Rawal-87, Chakwal-86 and Pak-81. At vegetative stress genotype Pak-81 showed lower MDA content with elevated levels of superoxide dismutase. Under anthesis and vegetative + anthesis stress, Rawal-87 and Chakwal-86 showed lower levels of MDA, peroxidase and superoxide dismutase. Kohistan-97, Inqilab-91, Watan and Uqaab-2000 showed pronounced lipid peroxidation with low activity of peroxidase and superoxide dismutase at different irrigation levels. These levels reflect the susceptibility of the genotypes towards water stress. This variable response of genotypes can be related to differences in antioxidant enzyme levels and could be used for selecting genotypes that are stress tolerant.

Keywords:

Wheat, Triticum aestivum L., water stress, MDA content, POD, SOD

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Journal: Journal of Food, Agriculture and Environment
Year: 2010
Volume: 8
Issue: 2
Category: Agriculture
Pages: 521-526

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