Journal of Food, Agriculture and Environment

Vol 8, Issue 2,2010
Online ISSN: 1459-0263
Print ISSN: 1459-0255

Multiresidue method of analysis for determination of 150 pesticides in grapes using quick and easy method (QuEChERS) and LC-MS/MS determination


Abd El-Moneim M. R. Afify 1*, Mahmoud A. Mohamed 1, Hassan A. El-Gammal 2*, Emad R. Attallah 2*

Recieved Date: 2009-12-20, Accepted Date: 2010-04-12


Multiresidue method for determination of 150 pesticides in grapes has been validated using quick, easy, cheap, effective, rugged and safe (QuEChERS) extraction method followed by liquid chromatography tandem mass spectrometry (LC-MS/MS) determination. The chosen pesticides are belonging to various chemical groups; they are registered in Egypt or most frequently detected in fruits and vegetables in Egyptain market. Grape samples are extracted by shaking with acetonitrile. Phase separation was induced by shaking with buffer–salt mixture consisting of magnesium sulfate, sodium chloride, disodium hydrogen citrate sesquihydrate and trisodium citrate dihydrate. The sample was centrifuged and an aliquot of the clear solution dried by shaking with magnesium sulphate. The extract was centrifuged and an aliquot of the clear solution evaporated, redissolved in methanol/water buffer solution and injected into LC-system. Quantitation and identity confirmation was attained by using atmospheric pressure electrospry positive ionization LC-MS/MS in multiple reaction monitoring (MRM) mode. The recoveries at three different concentration levels (0.01, 0.05 and 0.1 mg/kg) ranged from 70 to110%. The repeatability expressed as relative standard deviation ranged (RSDr ) was 1-25% (n=6). Matrix matched standards were used to compensate for the matrix effect.


Food analysis, pesticide residues, dispersive solid phase extraction, QuEChERS, grapes

Journal: Journal of Food, Agriculture and Environment
Year: 2010
Volume: 8
Issue: 2
Category: Agriculture
Pages: 602-606

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