Journal of Food, Agriculture and Environment

Assessment of genetic diversity in Trifolium spp. using ISSR and RAPD markers

Author(s):

Giedrė Dabkevičienė ^*, Vanda Paplauskienė, Egidijus Vilčinskas

Recieved Date: 2010-08-18, Accepted Date: 2011-01-10

Abstract:

Determination of genetic diversity in plants is the first step in developing breeding programmes. Many of the commercially valuable Trifolium species are used for forage. Recently attention has been drawn to other species characterised by useful traits, which could meet specific needs. This study estimates DNA polymorphism in the species not only used for forage (Trifolium pratense L. and Trifolium repens L.), but also in those promising in terms of nature conservation (T. medium L.) and organic farming (T. resupinatum L.). Polymorphism of the populations was studied using different marker systems: ISSR (Inter Simple Sequence Repeat) and RAPD (Random Amplified polymorphic DNA). The research findings suggest that both marker systems applied are suitable for clover DNA polymorphism studies. Polymorphic fragments were found to account for 72.1% and 68.6%, respectively. Using ISSR, the fragment size varied within a significantly narrower range (250-2500 bp) compared with that obtained by RAPD technique (150-5000 bp). Using ISSR markers, other indicators of polymorphism slightly exceed the values recorded for RAPD primers. Species-specific markers were identified. In our study, their number was similar for both marker systems (ISSR – 7.1 per primer; RAPD – 6.1).

Keywords:

Trifolium spp., ISSR, RAPD, polymorphism

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Journal: Journal of Food, Agriculture and Environment
Year: 2011
Volume: 9
Issue: 1
Category: Agriculture
Pages: 210-214

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