Journal of Food, Agriculture and Environment

Possibilities of reducing the variability of the development stages and the quality variability of the embryos obtained from embryo donor cows

Author(s):

Nicolae Păcală ^*, Ioan Bencsik, Dorel Dronca, Ioan Petroman, Cornelia Petroman, Ada Cean, Valeriu Caraba, Alexandra Boleman

Recieved Date: 2010-10-18, Accepted Date: 2011-01-04

Abstract:

The aim was to establish if adding the gestagen hormones to the superovulation treatment will improve the quality of the recovered embryos. The experiment was conducted on 12 Romanian Black and White and Romanian Spotted cows. The Synchro Mate-B (SMB, Intervet) implants, impregnated with 3 mg of Norgestomet, were used as gestagen hormones source. The implants were introduced subcutaneous, on the external face of the ear and there was administrated 2 ml injectable solution containing 3 mg Norgestomet and 2 ml injectable solution. The day on which the implants were introduced was considered the 0 day of the hormonal treatment. The superovulation started in the 5^th day by administrating FSH hormone (Folltropin-V, Vetrephorm, Canada Inc.), in a total dose of 400 mg NIH-FSH-P1, divided in 8 doses, each 50 mg, administrated at 12 hours interval. With the last two doses of FSH, 25 mg Lutalyse (PgF2µ) was administrated. The SMB implant was redrawn in the 9th day after the last FSH dose. The embryos were recovered in the 7^th day from the induced estrus. Embryo quality was evaluated after the morphological criteria described in IETS manual ⁶. The embryos were classified after the following quality codes: Code 1 (excellent quality), Code 2 (good quality), Code 3 (satisfactory quality) and Code 4 (unviable embryos). The mean number of viable embryos recovered per donor cow was 8.9. The majority of the embryos (68.9%) was in blastocyst developmental stage. The embryos were classified according to their quality as follows: Code 1 (37.1%), Code 2 (15.7%), Code 3 (12.4%) and Code 4 (34.8%). From the unviable embryos (Code 4) 51.6% were degenerated, 29.0% were retarded and 19.4% were unfertilized oocytes. We consider that the gestagen hormones are involved in increasing the proportion of viable embryo and lead to a reduction of the variability of the developmental stages, since the majority (68.9%) of the recovered embryos was in blastocyst stage.

Keywords:

Cow embryo, developmental stage, embryo quality, superovulatory treatment, FSH, Norgetsomet

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Journal: Journal of Food, Agriculture and Environment
Year: 2011
Volume: 9
Issue: 1
Category: Agriculture
Pages: 219-221

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