Journal of Food, Agriculture and Environment

Vol 9, Issue 1,2011
Online ISSN: 1459-0263
Print ISSN: 1459-0255

Biochemical and physiological factors related to cold de-acclimation and re-acclimation in rapeseed shoots in vitro


Natalija Burbulis 1*, Vaida Jonytienė 1, Ramunė Kuprienė 1, Aušra Blinstrubienė 1, Vytautas Liakas 2

Recieved Date: 2010-11-06, Accepted Date: 2011-01-12


Winter-hardiness is a complex trait limiting cultivation of winter rapeseed (Brassica napus L.) with respect to the regions of temperate climate. The de-acclimation resistance and re-acclimation capacity play a signicant role in determining plant hardiness during late winter and early spring. Development of freezing tolerance is based on complex metabolic and structural changes in plant cells involving regulation at various levels. The best-characterized changes include increases in soluble sugars, proteins, amino acids and other solutions. Therefore, this research was conducted to evaluate the changes in proline and soluble sugar levels during rapeseed shoots cold de-acclimation and re-acclimation in vitro. Investigations were carried out in the laboratory of Agrobiotechnology of Lithuanian University of Agriculture. Shoots of cultivar ‘Sunday’ were acclimated at 4°C for 14 days and subjected to de-acclimation under temperatures of 18/16ºC, photoperiod 16/8 h (day/night) for 1, 3, 5 and 7 days. After deacclimation, plants were subjected to re-acclimation at 4°C for 1, 3 and 5 days. The membrane injury for leaf discs under freezing stress was estimated by the electrolyte leakage test. Exposure of cold-acclimated shoots to normal growth temperature (18/16ºC) significantly reduced cold tolerance as well as soluble sugars and proline content. Re-acclimation of rapeseed shoots at 4°C resulted in a gradual increase in soluble sugars and proline contents. When de-acclimation duration was not longer than 5 days, rapeseed shoots of tested cultivar were able to recover cold tolerance to a level near or higher as before the de-acclimation treatment after re-exposure to 4°C temperature for 5 days.


Brassica napus, cold de-acclimation, cold re-acclimation, cold tolerance, proline, soluble sugars

Journal: Journal of Food, Agriculture and Environment
Year: 2011
Volume: 9
Issue: 1
Category: Environment
Pages: 483-487

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