Journal of Food, Agriculture and Environment

Vol 9, Issue 1,2011
Online ISSN: 1459-0263
Print ISSN: 1459-0255

Plant regeneration in inflorescence culture of chrysanthemum (Dendranthema x grandiflora (Ramat.) Kitamura)


Danuta Kulpa

Recieved Date: 2010-09-17, Accepted Date: 2011-01-20


The aim of this study was to determine the effects of initial explants, growth regulators and light on the organogenesis in the cultures of chrysanthemum inflorescence parts. Ray flowers, disc florets and flower bottom of Erica variety of chrysanthemum were used for initiation of culture. Explants placed on the MS medium supplemented with auxins (1.0 mg·dm-3 IAA, IBA, NAA and 2.4-D) or with a combination of auxin NAA (from 1.0 to 5.0 mg·dm-3) and cytokinine BAP (from 0.1 to 2.0 mg·dm-3). Half of the cultures were carried out in the light of the intensity 50 PAR while the other half in the dark. The obtained results showed that ‘Erica’ can be successfully regenerated in vitro culture from inflorescence parts. Disc florets were found to have the poorest regeneration ability under experimental conditions and the greatest ability was observed in the case of receptacle. The highest number of regenerated plants was obtained, irrespective of the used initial explants on MS medium with BAP (2 mg·dm-3) and an auxin NAA (5 mg·dm-3). Light stimulated plant regeneration in all experimental combinations.


Dandranthema, inflorescence culture, growth regulators, light

Journal: Journal of Food, Agriculture and Environment
Year: 2011
Volume: 9
Issue: 1
Category: Environment
Pages: 715-718

Full text for Subscribers

Note to users

The requested document is freely available only to subscribers/registered users with an online subscription to the Journal of Food, Agriculture & Environment. If you have set up a personal subscription to this title please enter your user name and password. All abstracts are available for free.

Article purchasing

If you like to purchase this specific document such as article, review or this journal issue, contact us. Specify the title of the article or review, issue, number, volume and date of the publication. Software and compilation, Science & Technology, all rights reserved. Your use of this website details or service is governed by terms of use. Authors are invited to check from time to time news or information.

Purchase this Article:   20 Purchase PDF Order Reprints for 15

Share this article :