Journal of Food, Agriculture and Environment




Vol 9, Issue 2,2011
Online ISSN: 1459-0263
Print ISSN: 1459-0255


Hyperglucidic stress in diet – study of the dentine-pulp complex on experimental model


Author(s):

Roxana Oancea 1*, Angela Codruţa Podariu 1, Liliana Vasile 2, Valentin Ordodi 3, Cristian Oancea 4

Recieved Date: 2011-01-08, Accepted Date: 2011-04-12

Abstract:

High glucose quantity accelerates the initialization and progression of dental caries, but it seems also to affect dentin formation in young rats. Based on the hypothesis that metabolical alterations of odontoblasts might predispose to caries, evidenced through the decrease in dentinal apposition, it was assumed that glucose can contribute to the progression of carious lesion through systemic mechanism. The reactivity of the pulp-dentinal complex on experimental model was investigated in increased carious environment, modifying the diet (high glucose concentration) and the salivary function (ablation of the submandibular salivary gland). In order to analyse the carious mechanism, experimental model was created. Sprague-Dawley rats aged 21 days were used in this experiment. All the animals were injected intraperitoneally with oxytetracycline (30-40 mg/kg) to mark the dentin areas formed during the study. The first molar erupts into the oral cavity on the 19th day after birth and reaches the occlusal plane on day 25, while the second molar erupts on the 22nd day and becomes functional on the 28th day. Control group consisted of feeding rats with standardized protein concentrate with no surgery. Group 2 consisted of feeding rats with the same standardized protein concentrate, with excision of submandibular salivary glands. Group 3 consisted of feeding rats with food made from standard protein concentrate (40%) and glucose (60%), with no surgery. Group 4 consisted of feeding rats with the same food as group 3 and excision of submandibular glands. Samples from 4 groups including 4 rats were collected after ethical approval. The smears were analysed by cytohistological methods. On the experimental model, the effect of the salivary submandibular ablation and the high glucose concentration diet was the increasing carious activity. The high glucose concentration diet reduced mineralised dentinal apposition in young rat. Dentin formation was smaller and the predentin zone wider in rats fed a glucose diet when compared with rats fed the reference diet. Widening of the predentin zone in rats fed a high-glucose diet may reflect changes in odontoblast function, such as reduced matrix synthesis and possibly disturbed mineralization. In the control group the dentinal apposition was significantly higher under the carios lesion. The high dose of glucose in diet decreased dentinal formation in comparison to the control group. The control group showed a defensive pulp- dentinal response while the carious diet group showed a decrease of the pulp-dentinal response. These aspects reveal the importance of diet in modulating the response against progression of dental caries.

Keywords:

High glucose concentration, diet, experimental model, pulp-dentinal response


Journal: Journal of Food, Agriculture and Environment
Year: 2011
Volume: 9
Issue: 2
Category: Food and Health
Pages: 152-157


Full text for Subscribers
Information:

Note to users

The requested document is freely available only to subscribers/registered users with an online subscription to the Journal of Food, Agriculture & Environment. If you have set up a personal subscription to this title please enter your user name and password. All abstracts are available for free.

Article purchasing

If you like to purchase this specific document such as article, review or this journal issue, contact us. Specify the title of the article or review, issue, number, volume and date of the publication. Software and compilation, Science & Technology, all rights reserved. Your use of this website details or service is governed by terms of use. Authors are invited to check from time to time news or information.


Purchase this Article:   20 Purchase PDF Order Reprints for 15

Share this article :