Journal of Food, Agriculture and Environment

Using amplified fragment length polymorphism marker to investigate the genetic diversity of local and wild wheat in Syria

Author(s):

Rami Khalil ^{1, 2}, Zhengguo Li ^1*

Recieved Date: 2011-01-08, Accepted Date: 2011-03-29

Abstract:

In this study, the genetic relationship between T. durum, T. aestivum, T. dicoccoides and T. urartu was estimated by using Amplified Fragment Length Polymorphism (AFLP) markers. EcoRI and MseI were the selective primers used with one nucleotide extention on the 3’ end. The number of unique markers per genotype ranged from 1 to 17, the highest one was revealed by T. dicoccoides while the lowest one by T. durum. The relationship between the wheat varieties was obtained. The 11 AFLP primer combinations amplified 657 AFLP loci of which 594 (90.4%) were polymorphic. Based on cluster analysis the most distant two cultivars are Senaljamal (Se) (T. durum) and U1 (T. urartu), and the closest two are Bosiedi (Bo) and Aiubieh (Ai).

Keywords:

Wheat (T. durum,T. aestivum,T. dicoccoides,T. urartu), landraces, genetic relationship, AFLP, cluster analysis

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Journal: Journal of Food, Agriculture and Environment
Year: 2011
Volume: 9
Issue: 2
Category: Agriculture
Pages: 345-349

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