Journal of Food, Agriculture and Environment

Preconcentration and separation of copper, zinc and lead ions on Geobacillus stearothermophilus DSMZ 22 loaded on silica gel

Author(s):

Gülşen Güven ¹, Mustafa Demir ^1*, Gamze Başbülbül ²

Recieved Date: 2011-02-09, Accepted Date: 2011-04-12

Abstract:

In this work, a method was developed for Cu (II), Zn (II) and Pb (II) preconcentration by using columns packed with Geobacillus stearothermophilus DSMZ 22 (thermophilic bacterial biomass) immobilized on silica gel. The thermophilic bacterium G. stearothermophilus DSMZ 22 immobilized on silica gel was used as a new biosorbent for the preconcentration and separation of copper, zinc and lead ions. G. stearothermophilus DSMZ 22 immobilized on silica gel selectively accumulated analytes from water samples. The retained copper, zinc and lead ions on the biosorbent were eluted by using 1 M HCl and analysed by ICP-OES. The influences of analytical parameters including pH, amount of bacterial biomass, sample volume, eluent volume, type of eluent etc. on the quantitative recoveries of analytes were investigated. The effects of some alkaline, alkaline earth and some metal ions on the recoveries of analytes were also examined. The maximum sorption capacity of the biosorbent under the optimized experimental conditions was 1630 µg g^-1 for Zn (II). The detection limit for Cu (II), Pb (II) and Zn (II) ions were 1.51, 3.11 and 4.48 µg l^-1, respectively. In order to validate the accuracy of the method for determination of copper, zinc and lead, certified reference material of sewage sludge (BCR 146-R) was used. Proposed separation/ preconcentration method was applied to spring, tap and river water samples.

Keywords:

Preconcentration, biosorption, Geobacillus stearothermophilus, silica gel, trace metals

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Journal: Journal of Food, Agriculture and Environment
Year: 2011
Volume: 9
Issue: 2
Category: Environment
Pages: 607-611

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