Journal of Food, Agriculture and Environment

Characterization and content of total soluble protein and amino acids of traditional common bean cultivars collected in Parana state, Brazil

Author(s):

Pedro S. Vidigal Filho ¹, Maria C. Gonçalves-Vidigal ¹, Andréa B. da Rocha ², Ray Hammerschmidt ², William W. Kirk ², Juliana Parisotto Poletine ^1*, James D. Kelly ³

Recieved Date: 2011-04-20, Accepted Date: 2011-09-30

Abstract:

Twenty-five traditional landrace cultivars of common bean (Phaseolus vulgaris L.) from Parana State, Brazil, representing five commercial seed classes, Carioca, Preto, Navy, Rosinha and Manteigão, were analyzed for total soluble protein and amino acid contents. Total soluble protein content ranged from 11.5 to 36% and the total soluble amino acids ranged from 1163 to 2120 µmol.g^-1. When total protein was separated by gel electrophoresis, bands corresponding to molecular weights of 249 and 264 bp (S phaseolin type) were observed for 17 cultivars. In the case of T phaseolin, three bands (246 to 300 bp) were detected for eight cultivars. Results demonstrated that among the 25 cultivars tested, eight showed T phaseolin (Andean) and 17 S phaseolin type (Mesoamerican). Data from this study would suggest that genetic variability observed in total protein and soluble amino acid contents in the 25 traditional bean cultivars from Brazil may be used to enhance protein content of future common bean cultivars. These common bean cultivars also possess genes that could be useful in breeding programs to enhance resistance to Andean and Mesoamerican races of Colletotrichum lindemuthianum.

Keywords:

Grain legumes, protein characterization, polyacrylamide gel electrophoresis

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Journal: Journal of Food, Agriculture and Environment
Year: 2011
Volume: 9
Issue: 3&4
Category: Food and Health
Pages: 143-147

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