Journal of Food, Agriculture and Environment
Vol 9,
Issue 3&4,2011
Online ISSN: 1459-0263
Print ISSN: 1459-0255
|
|
|
|
Newsletter
Subscribe to our newsletter and stay up to date with the latest publishes
Variations in bioactivity and conformation of trypsin adsorbed on different size nanoparticles
Author(s):
Li Li,
Hong-Hua Xu *, Yu-Jun Jiang, Guo-Ping Yu, Li-Gang Zhang, Yan XuRecieved Date: 2011-02-10, Accepted Date: 2011-10-06
Abstract:The variations of conformation and enzyme activity of trypsin adsorbed on SiO2 particles of various sizes (20 nm, 100 nm, 300 nm and amorphous level) had been studied. The adsorption amount of trypsin clearly decreased with the increase of SiO2 particle sizes. The maximal adsorption amount was on 20 nm SiO2 at pH 7.8, and the relative activity of trypsin adsorbed on SiO2 of 20 nm, 100 nm, 300 nm and amorphous level at the same conditions was 61.3%, 56.3%, 43.5% and 23.2%, respectively. Both enzyme stability and protein structure were strongly dependent on the size of the particles. Smaller SiO2 particles enhanced trypsin stabilities in strongly denaturing environments to a greater extent than that of larger ones. When the SiO2-trypsin bioconjugates incubated at 80°C for 1h, the activity for trypsin adsorbed onto 20 nm, 100 nm, 300 nm and amorphous particles increased 96.3%, 70.8%, 45.7% and 19.7% of its native activity, respectively. The fraction of activity lost correlated well with the increase of surface hydrophobicity, and greater increase in surface hydrophobicity was observed for the trypsin adsorbed onto larger nanoparticles under otherwise similar conditions. DSC measurement also confirmed that the size of the nanoparticles influenced the attachment of protein structure and thermal stability.
Keywords:Trypsin,
SiO2 particles, adsorption, enzyme activity, surface hydrophobicity, DSC measurementJournal: Journal of Food, Agriculture and Environment
Year: 2011
Volume: 9
Issue: 3&4
Category: Food and Health
Pages: 167-171
Full text for Subscribers
Information:
Note to users
The requested document is freely available only to subscribers/registered users with an online subscription to the Journal of Food, Agriculture & Environment. If you have set up a personal subscription to this title please enter your user name and password. All abstracts are available for free.
Article purchasing
If you like to purchase this specific document such as article, review or this journal issue, contact us. Specify the title of the article or review, issue, number, volume and date of the publication. Software and compilation, Science & Technology, all rights reserved. Your use of this website details or service is governed by terms of use. Authors are invited to check from time to time news or information.
Purchase this Article: 20 Purchase PDF Order Reprints for 15
Share this article :