Journal of Food, Agriculture and Environment

Multiplication and cryogenic storage of Artemisia herba-alba: A medicinal plant

Author(s):

Mohamad A. Shatnawi

Recieved Date: 2011-06-10, Accepted Date: 2011-10-04

Abstract:

An efficient in vitro propagation for A. herba-alba was developed as an alternative to seed propagation. Maximum number of shoots (5.25 new shoot/ explant) was obtained on Murashige and Skoog (MS) supplement with 0.6 mg/L BAP (benzyl amino purine). The shoots were easily elongated as well as rooted on MS medium supplemented with indole-3-butyric acid (IBA), indole acetic acid (IAA) or naphthalene acetic acid (NAA) at various concentrations. The plantlets were ex vitro acclimatized in pots containing a mixture of sand, peat; perlite (1:1:1) and gave 70% survival. Excised shoot tips from cold-hardened plantlets at 6±2ºC, with a photoperiod of 16 h light/8 h dark, were precultured for one day on solid MS medium containing 0.3 M sucrose, in the dark. The precultured shoot tips were trapped into alginate gel beads and treated in medium supplemented with 0.75 M sucrose for one day. The beads containing shoot tips were dehydrated under a laminar airflow cabinet at 24±2ºC to 21% water content (fresh weight basis) before being stored in liquid nitrogen (LN) at -196°C. The average rate of shoot formation after thawing was 70%. Thus, simple in vitro propagation method and cryogenic storage of A. herba-alba was successfully developed with study.

Keywords:

Artemisia herba-alba, cryogenic storage, cold hardening, encapsulation dehydration, germplasm conservation, sodium alginate

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Journal: Journal of Food, Agriculture and Environment
Year: 2011
Volume: 9
Issue: 3&4
Category: Agriculture
Pages: 340-344

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