Journal of Food, Agriculture and Environment

Vol 9, Issue 3&4,2011
Online ISSN: 1459-0263
Print ISSN: 1459-0255

Embryogenesis and plant regeneration from isolated microspores of Brassica napus L. under different incubation time


Behzad Ahmadi 1, Mortaza Ghadimzadeh 2, Amir Fayaz Moghaddam 2, Khoshnood Alizadeh 3*

Recieved Date: 2011-07-11, Accepted Date: 2011-09-30


Effects of bud length (2-3 and 4-5 mm) and incubation time (4, 8 and 12 days) at 30°C on microspore embryogenesis were evaluated on winter rapeseed cv. Amica. Furthermore, the effect of gibberellic acid (GA3) and effects of different concentrations and factorial combination of benzyladenine (BA) and indole-3-acetic acid (IAA) were studied as two individual trials on embryo regeneration of rapeseed microspore. Microspore embryogenesis was affected significantly by bud length and heat shock incubation time. The highest level of embryogenesis was obtained from 2-3 mm bud length for 12 days of incubation at 30°C. The highest percentage of direct regeneration of embryos was observed in embryos with 4-6 mm length using 0.2 mg/l BA + IAA 0.0 mg/l. Also, the highest percentage of direct regeneration was related to embryos 4-6 mm in length cultured on medium supplemented with GA3 0.05 mg/l. Our results revealed that embryogenesis and plant regeneration of rapeseed could be improved by choosing proper bud length as well as appropriate incubation treatment and using different phytohormone levels.


Doubled haploid, embryogenesis, heat treatment, plant regeneration rapeseed

Journal: Journal of Food, Agriculture and Environment
Year: 2011
Volume: 9
Issue: 3&4
Category: Agriculture
Pages: 434-437

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