Journal of Food, Agriculture and Environment

Genome shuffling enhanced antagonistic activity against Fusarium oxysporum f. sp. melonis and tolerance to chemical fungicides in Bacillus subtilis BS14

Author(s):

Liang Chen, Wuling Chen ^*

Recieved Date: 2009-01-19, Accepted Date: 2009-04-17

Abstract:

Genome shuffling was employed for a biocontrol strain BS14 to enhance antagonistic activity against Fusarium oxysporum f. sp. melonis (FOM) and tolerance to two chemical fungicides. Strain BS14 was identified as a strain of Bacillus subtilis by the analysis of 16S rDNA sequences. A stable recombinant F35 was obtained after three rounds of shuffling. Antagonistic activity of recombinant F35 against FOM was increased by 34.52% and 65.48% compared to that of the parent strain HN8-7 with highest activity and another parent strain utilized, BS14. The tolerance to chemical fungicides was also significantly improved (p ≤ 0.05) compared to that of strain BS14. Reduction of FOM of 94% was observed by using recombinant F35, which was increased by 45% compared to that of strain BS14 (p ≤ 0.05) and no significant differences (p > 0.05) compared to that of thiophanate methyl (MRL). Reduction of FOM of 100% was dramatically observed by using an integrated treatment combining MRL (50% of usual dosage) with recombinant F35. Strain F35 with these improved traits would be a promising biocontrol agent in the control of FOM. Here genome shuffling was proved to be a practical methodology for strain improvement of antagonistic microorganism Bacillus subtilis BS14 for enhancing antagonistic activity against FOM and tolerance to chemical fungicides.

Keywords:

Antagonistic activity, Bacillus subtilis, fungicide tolerance recombinant, genome shuffling, protoplast fusion

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Journal: Journal of Food, Agriculture and Environment
Year: 2009
Volume: 7
Issue: 2
Category: Environment
Pages: 856-860

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