Journal of Food, Agriculture and Environment

Optimization of culture conditions for high-level expression of dextransucrase in Escherichia coli

Author(s):

Hong-Bin Zhang ^*, Xiao-Qing Mao, Ya-Jie Wang, Xue-Qin Hu

Recieved Date: 2009-07-18, Accepted Date: 2009-10-08

Abstract:

The use of lactose as inducer for the expression of dextransucrase was investigated in high density of Escherichia coli. Different culture conditions including carbon source, nitrogen sources, inorganic salts, inoculum density (OD₆₀₀) and lactose concentrations were optimized, and high-level expression of dextransucrase in E. coli was achieved via an optimal culture conditions. The results indicated that glycerol and tryptone were suitable carbon source and nitrogen source for dextransucrase expression respectively; organic nitrogen sources were found to enhance the enzyme production. Optimal conditions were added lactose of 5 g l^-1 when OD₆₀₀ reached 3.0 and cultivation continued at 25°C for 7 h. Maximal activity under these conditions was 60.18 U/ml.

Keywords:

Dextransucrase, optimization, culture conditions, expression, lactose

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Journal: Journal of Food, Agriculture and Environment
Year: 2009
Volume: 7
Issue: 3&4
Category: Food and Health
Pages: 75-78

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