Journal of Food, Agriculture and Environment

Optimum extraction and high-throughput detection of cordycepic acid from medicinal macrofungi Cordyceps jiangxiensis, Cordyceps taii and Cordyceps gunnii

Author(s):

Jian-Hui Xiao ^{1, 2*}, Dai-Min Xiao ², Qing Xiong ², Zong-Qi Liang ², Jian-Jiang Zhong ^{1, 3*}

Recieved Date: 2009-06-30, Accepted Date: 2009-10-02

Abstract:

Cordycepic acid is a major metabolite of Cordyceps sinensis and used as one of markers for its quality control in China, and possesses interesting therapeutic activities. In this study, an optimized extraction condition for cordycepic acid from medicinal macrofungi C. jiangxiensis, C. taii and C. gunnii was established by an orthogonal design, including extraction time (1 h), temperature(40°C), extractant (ultrapure water), and frequency (twice). Based on higher specificity and high-throughput detecting capability of microplate reader, a new universal microplate reader assisted ultraviolet-visible spectrophotometry (UVS) at 412 nm for quantitative determination of cordycepic acid in Cordyceps species was developed. The linearity of microplate reader assisted UVS assay was found within 6.3-60 μg/ml concentration ranges of cordycepic acid, and the linear correlation coefficient was high (R²>0.9997) within the test ranges. The limits of detection (LOD) and quantification (LOQ) were 1.8 μg/ml and 6.3 μg/ml, respectively. The recoveries were in the range of 96.71 to 104.61%. With the developed method, the yield of cordycepic acid from C. jiangxiensis, C. taii and C. gunnii was 11.81%, 8.72% and 4.73% of the dry weight of mycelium, respectively. Taken together, comparing to conventional UVS, the microplate reader assisted UVS method showed good accuracy, less error, and high-throughput detecting advantage, and it is considered as an alternative useful and effective technique for the fast determination of large number of analytes. In addition, as a marker of quality control in Cordyceps bioproduct, quantitative determination of cordycepic acid will also contribute to a rational quality control standard system for the crude drugs and bioproducts of C. jiangxiensis, C. taii and C. gunnii.

Keywords:

Cordycepic acid, microplate reader assisted ultraviolet-visible spectrophotometry, Cordyceps, quantitative analysis, medicinal herb

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Journal: Journal of Food, Agriculture and Environment
Year: 2009
Volume: 7
Issue: 3&4
Category: Agriculture
Pages: 328-333

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