Journal of Food, Agriculture and Environment
Vol 10,
Issue 1,2012
Online ISSN: 1459-0263
Print ISSN: 1459-0255
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Optimization of medium composition for production of lacticin LLC518 by Lactococcus lactis subsp. Lactis LLC518 using response surface methodology
Author(s):
Qinglong Meng,
Qingxia Cai, Bo Shi, Ruiyan Fu, Jinnian Li, Xiaolin Chen, Kezong Qi, Ming Zhang *Recieved Date: 2011-09-10, Accepted Date: 2012-01-08
Abstract:Response surface methodology (RSM) was applied for the optimization of lacticin LLC518 production, which was produced by Lactococcus lactis subsp. lactis LLC518. Tryptone, KH2PO4 and MgSO4 had signicant effects on lacticin LLC518 biosynthesis by a Plackett-Burman design (PBD). After steepest ascent design (SAD) and central composite design (CCD), the optimum medium compositions were obtained as follows (g/l): sucrose 20, yeast extract 5, tryptone 8.18, beef extract 10, MgSO4·7H2O 0.82, K2HPO4 2.8, (NH4)2HC6H5O7 5.0, CH3COONa 5.0, MnSO4 0.25 and Tween 80 5 ml. An 86.3% increase in bacteriocin production (180.13 IU/ml) was achieved under this condition compared within unoptimized medium (96.72 IU/ml). Under these optimized conditions, the production of Lacticin LLC518 began at 4 h, peaked at 14 h.
Keywords:Optimization,
bacteriocin, Lactococcus lactis, lacticin, response surface methodologyJournal: Journal of Food, Agriculture and Environment
Year: 2012
Volume: 10
Issue: 1
Category: Food and Health
Pages: 137-142
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