Journal of Food, Agriculture and Environment

Vol 7, Issue 3&4,2009
Online ISSN: 1459-0263
Print ISSN: 1459-0255

Morpho-physiological evaluation and RAPD markers-assisted characterization of date palm (Phoenix dactylifera L.) varieties for salinity tolerance


Shyam S. Kurup, Yousef S. Hedar, Mouza A. Al Dhaheri, Ahmed Y. El-Heawiety, Mohammed A. M. Aly *, Ghaleb Alhadrami

Recieved Date: 2009-05-17, Accepted Date: 2009-10-10


Date palm (Phoenix dactylifera L.) is one of the important fruit crops in arid land. The conservation of date palm genetic diversity is thus an important task to maintain the genetic resources. In order to identify salinity tolerant date palms, evaluation of four varieties was conducted based on analysis of morphological and physiological parameters, e.g. growth and pigment analysis, respectively. Randomly amplified polymorphic DNA (RAPD) technique was employed to characterize the varieties. The varieties were irrigated with increasing concentrations of saline water from 7.8, 11.7, 15.6, 19.5, 23.4 and 27.3 dS/m at regular intervals of three months duration. The growth parameters were plant height, collar girth and number of leaves. The physiological parameters were the types and quantities of the photosynthetic pigments. The varieties responded differentially to the increasing salt concentration with Bugal White acquiring maximum plant height, collar girth and production of leaves. The sensitivity to high salinity recorded reduced growth, leaf production and collar girth in varieties Mesalli and Razez. Chlorophyll and carotenoid contents of the treated varieties were highest in the varieties Bugal White and Khashkar and significantly superior to Mesalli and Razez. The date palm varieties were fingerprinted using fourteen random primers to detect the DNA polymorphism. Primer OPC-02 revealed a 1400 bp fragment amplified in ‘Bugal White’ (salinity tolerant) and ‘Khlas’ which is known to be drought tolerant. Primer OPD-02 distinguished ‘Bugal White’, which proved to be salinity tolerant, with a DNA fragment of about 1200 bp. Primer OPD-02 amplified a 1600 bp fragment in ‘Khashkar’, ‘Bugal White’, ‘Shaham’ and ‘Khlas’.


Phoenix dactylifera, RAPD markers, salinity tolerance, morpho-physiology, pigments

Journal: Journal of Food, Agriculture and Environment
Year: 2009
Volume: 7
Issue: 3&4
Category: Agriculture
Pages: 503-507

Full text for Subscribers

Note to users

The requested document is freely available only to subscribers/registered users with an online subscription to the Journal of Food, Agriculture & Environment. If you have set up a personal subscription to this title please enter your user name and password. All abstracts are available for free.

Article purchasing

If you like to purchase this specific document such as article, review or this journal issue, contact us. Specify the title of the article or review, issue, number, volume and date of the publication. Software and compilation, Science & Technology, all rights reserved. Your use of this website details or service is governed by terms of use. Authors are invited to check from time to time news or information.

Purchase this Article:   20 Purchase PDF Order Reprints for 15

Share this article :