Journal of Food, Agriculture and Environment
Vol 8,
Issue 2,2010
Online ISSN: 1459-0263
Print ISSN: 1459-0255
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Molecular cloning, segmental distribution and ontogenetic expression of the amino acid transporter y+LAT1 in intestine of the Tibetan suckling piglets
Author(s):
Wanting Gu 2,
Jun Fang 1*, Wence Wang 2, Ming Fan 3, Meimei Geng 2, Teijun Li 2, Wuyin Chu 4Recieved Date: 2009-10-17, Accepted Date: 2010-04-10
Abstract:The y+LAT1 gene is a member of solute carrier family that encodes a protein, which plays a significant function in transport of neutral and dibasic amino acids. The Tibetan pig is an excellent local variety in China, which is able to tolerate and survive under the adverse conditions. The study cloned the cDNA sequence of Tibetan pig y+LAT1 (GenBank accession #EU390782), and evaluated the tissue distribution and developmental expression of y+LAT1 mRNA and protein in different parts of intestine in Tibetan suckling piglets. The results show that the y+LAT1 cDNA encodes 511 deduced amino acid residues and 12 putative transmembrane domains, which can be widely detected in many organs in Tibetan piglets. Expression patterns were similar in jejunum and ileum, where the mRNA and protein level was decreased before the suckling period and increased until Day 35, duodenum changed reversely. And the jejunum posterior is the predominant expressional tissue in all days. These results indicated that the ontogenesis expression of y+LAT1 was not only differentially regulated by early development age but also differentially with distributed tissues along the small intestine.
Keywords:Amino acid transporter,
y+LAT1, Tibetan suckling piglets, ontogenetic expressionJournal: Journal of Food, Agriculture and Environment
Year: 2010
Volume: 8
Issue: 2
Category: Environment
Pages: 1067-1072
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