Journal of Food, Agriculture and Environment

Vol 10, Issue 2,2012
Online ISSN: 1459-0263
Print ISSN: 1459-0255

Listeria monocytogenes in cig kofte without meat: A novel bulgur ball product


Birce Mercanoglu Taban

Recieved Date: 2012-01-06, Accepted Date: 2012-04-10


In this study, the presence of Listeria monocytogenes in cig kofte without meat was analysed. Seventy samples were purchased from various markets and retail centres in Ankara over an 8-month period. Bacterial colonies suspected to be L. monocytogenes were isolated by a method that begins with a primary enrichment in Half-Fraser broth followed by immunomagnetic separation (IMS) with magnetic beads coated with an antibody against L. monocytogenes and plating on PALCAM and Oxford agars. Subsequent selective identification on Harlequin™ Listeria Medium and polymerase chain reaction (PCR) confirmation were used. The PCR method used a primer pair targeted at a specific region of the internalin (inlA) gene of L. monocytogenes, giving a product of 250 bp. Detection of L. monocytogenes in the 70 samples with IMS and PCR primers specific to the inlA gene that encodes the virulence of L. monocytogenes showed that 17.1% (12 of 70) samples contained L. monocytogenes. The inlA gene was detected in 12 L. monocytogenes isolates obtained from different cig kofte samples. The data gathered in this study are important for the assessment of the risk of listeriosis posed by L. monocytogenes in cig kofte, even when it is prepared without meat. Consumers should be warned of the health risks associated with eating such raw foods to protect the public from listeriosis. 


Contamination, cig kofte, IMS, inlA, Listeria monocytogenes, novel food, PCR, public health, raw food, ready-to-eat (RTE) food

Journal: Journal of Food, Agriculture and Environment
Year: 2012
Volume: 10
Issue: 2
Category: Food and Health
Pages: 130-132

Full text for Subscribers

Note to users

The requested document is freely available only to subscribers/registered users with an online subscription to the Journal of Food, Agriculture & Environment. If you have set up a personal subscription to this title please enter your user name and password. All abstracts are available for free.

Article purchasing

If you like to purchase this specific document such as article, review or this journal issue, contact us. Specify the title of the article or review, issue, number, volume and date of the publication. Software and compilation, Science & Technology, all rights reserved. Your use of this website details or service is governed by terms of use. Authors are invited to check from time to time news or information.

Purchase this Article:   20 Purchase PDF Order Reprints for 15

Share this article :