Journal of Food, Agriculture and Environment

An efficient regeneration system for peanut: Somatic embryogenesis from embryonic leaflets

Author(s):

Mingxia Zhao, Jiongming Sui, Lixian Qiao, Lingling Tan, Jingshan Wang *

Recieved Date: 2012-01-17, Accepted Date: 2012-04-22

Abstract:

An efficient method of plant regeneration via somatic embryogenesis was developed for five peanut cultivars. Somatic embryogenesis was induced from embryonic leaflets on MSB5 medium (Murashige and Skoog salts plus B₅ vitamins) supplemented with different concentrations of 2,4- dichlorophenoxyacetic acid (2,4-D). After 3 weeks, the frequency of somatic embryo formation was highest with 5 mg L^-1 2,4-D for cultivar Huayu 23 (frequency 76.1%), 5 or 10 mg L^-1 2,4-D for cultivar Huayu 20 (~ 79.5%), or 10 mg L^-1 2,4-D for cultivars Huayu 22, Huayu 25, and Luhua 11 (92.9, 91.3, and 92.3%, respectively). When explants with somatic embryos were subcultured on a germination medium, conversion of somatic embryos into plantlets was highest with 6-benzylaminopurine (BAP; 74.6–93.4% conversion) and next highest with thidiazuron (35.6–78.2%, depending on concentration) but was substantially lower with gibberellic acid (0–24.6%, depending on concentration), abscisic acid (0–3.9%, depending on concentration), or the control (without growth regulators; 3.2–23.8%). When 33 regenerated Huayu 23 plantlets were cleft grafted onto germinated seedlings and transplanted into the field, 31 grew to maturity and produced viable seeds.

Keywords:

Peanut (Arachis hypogaea L.), embryonic leaflet, somatic embryogenesis, regeneration

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Journal: Journal of Food, Agriculture and Environment
Year: 2012
Volume: 10
Issue: 2
Category: Agriculture
Pages: 527-531

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