Journal of Food, Agriculture and Environment

Vol 10, Issue 2,2012
Online ISSN: 1459-0263
Print ISSN: 1459-0255

An efficient regeneration system for peanut: Somatic embryogenesis from embryonic leaflets


Mingxia Zhao, Jiongming Sui, Lixian Qiao, Lingling Tan, Jingshan Wang *

Recieved Date: 2012-01-17, Accepted Date: 2012-04-22


An efficient method of plant regeneration via somatic embryogenesis was developed for five peanut cultivars. Somatic embryogenesis was induced from embryonic leaflets on MSB5 medium (Murashige and Skoog salts plus B5 vitamins) supplemented with different concentrations of 2,4- dichlorophenoxyacetic acid (2,4-D). After 3 weeks, the frequency of somatic embryo formation was highest with 5 mg L-1 2,4-D for cultivar Huayu 23 (frequency 76.1%), 5 or 10 mg L-1 2,4-D for cultivar Huayu 20 (~ 79.5%), or 10 mg L-1 2,4-D for cultivars Huayu 22, Huayu 25, and Luhua 11 (92.9, 91.3, and 92.3%, respectively). When explants with somatic embryos were subcultured on a germination medium, conversion of somatic embryos into plantlets was highest with 6-benzylaminopurine (BAP; 74.6–93.4% conversion) and next highest with thidiazuron (35.6–78.2%, depending on concentration) but was substantially lower with gibberellic acid (0–24.6%, depending on concentration), abscisic acid (0–3.9%, depending on concentration), or the control (without growth regulators; 3.2–23.8%). When 33 regenerated Huayu 23 plantlets were cleft grafted onto germinated seedlings and transplanted into the field, 31 grew to maturity and produced viable seeds.


Peanut (Arachis hypogaea L.), embryonic leaflet, somatic embryogenesis, regeneration

Journal: Journal of Food, Agriculture and Environment
Year: 2012
Volume: 10
Issue: 2
Category: Agriculture
Pages: 527-531

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