Journal of Food, Agriculture and Environment

Vol 8, Issue 3&4,2010
Online ISSN: 1459-0263
Print ISSN: 1459-0255

Bacterial contamination in lettuce: Comparison of sewage water-irrigated plants to commercial products from Riyadh market, Saudi Arabia


Omar M. H. Shair

Recieved Date: 2010-08-20, Accepted Date: 2010-11-08


The reuse of waste water in agriculture and aquiculture was sensitive subject. Chemicals and microbial contaminants in the sewage water stayed in the plant and were eaten by human in the food. The goal of this research was to study leaves of lettuce produced by seeding and watering with treated sewage water, sterilized distilled water and pipe water and lettuce from Riyadh market, Saudi Arabia. Germinated lettuce in planter and lettuce from market were grinded into pieces. Bacterial extraction buffer was added to the grinded samples, centrifuged and supernatants were smeared on nutrient agar in Petri dishes and incubated at 37ºC over night. Lettuce watered with treated sewage water was investigated for bacterial load. Bacterial colonies were observed, counted and compared with lettuce watered with distilled water, pipe water and lettuce from Riyadh market. The bacterial loads of lettuce produced with treated sewage water were higher than lettuce from Riyadh market and those produced with distilled and pipe water.


Bacterial load, distilled water, pipe water, lettuce, treated sewage water, Riyadh market, Saudi Arabia

Journal: Journal of Food, Agriculture and Environment
Year: 2010
Volume: 8
Issue: 3&4
Category: Agriculture
Pages: 470-472

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