Journal of Food, Agriculture and Environment

The mutation in nicotinic acetylcholine receptor β1 subunit may confer resistance to imidacloprid in Aphis gossypii (Glover)

Author(s):

Xu-Gen Shi ¹, Yu-Kun Zhu ¹, Xiao-Ming Xia ¹, Kang Qiao ¹, Hong-Yan Wang ², Kai-Yun Wang ^1,*

Recieved Date: 2012-02-18, Accepted Date: 2012-04-30

Abstract:

Neonicotinoid insecticides, such as imidacloprid, are selective agonists on the insect nicotinic acetylcholine receptors - their molecular target site, which are used extensively to control a variety of different pest species. Just like other classes of insecticides, resistance to neonicotinoids is a significant threat, which has been identified in several pest species, including the cotton aphid, Aphis gossypii (Glover), a major cotton pest in many parts of Asia. A 66.49-fold imidacloprid-resistant Aphis gossypii strain was established in our work after selection for 60 generations. Analysis of the cDNA sequence of the nicotinic acetylcholine receptor (nAChR) α1, α2, α3, α4-1,α4-2, β1 subunits and the functional extracellular region (ranging from loop A to the 1^st transmembrane domain) of the nicotinic acetylcholine receptor α5 subunit from the resistant strain revealed a single point mutation in the loop D region of the nAChR β1 subunit causing an arginine to threonine substitution (R81T). This mutation has been identified to be a key determinant of neonicotinoid binding to nAChRs and this amino acid change results in reduced sensitivity to neonicotinoids, which confers a vertebrate-like character to the insect nAChRs. This result indicated that in cotton aphids the single mutation (R81T) might confer imidacloprid resistance. 

Keywords:

Aphis gossypii (Glover), imidacloprid, resistance, mutation, nicotinic acetylcholine receptor

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Journal: Journal of Food, Agriculture and Environment
Year: 2012
Volume: 10
Issue: 2
Category: Environment
Pages: 1227-1230

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