Journal of Food, Agriculture and Environment

Preparation of a polyclonal antibody based heterologous indirect competitive ELISA for detecting ractopamine residue

Author(s):

Chao-ying Li ^{1, 2}, Jin-qing Jiang ²*

Recieved Date: 2011-12-10, Accepted Date: 2012-04-24

Abstract:

Based on the polyclonal antibody (pAb), an indirect competitive ELISA (icELISA) has been developed for the determination of ractopamine (Rac) residue in swine and cattle. Mixed anhydride method was employed to synthesize the immunogen while 1,4-butanediol diglycidyl ether was used to synthesize the coating antigen, thus producing the heterologous sensitivity; and two female New Zealand white rabbits were used to produce anti- Rac pAb. By the square matrix titration, an icELISA standard curve was established. The linear range was from 0.006 to 30 ng/mL, with LOD and IC₅₀ value of 0.003 and 0.44 ng/mL, respectively. Except for a moderate cross-reactivity (34.5%) to dobutamine, negligible cross-reactivity to other compounds tested was observed. After optimization, 1:10 dilution in swine muscle and 1:20 dilution in cattle muscle produced the satisfactory B₀ and IC₅₀ values, respectively. When applied in real sample tests, the correlation coefficients (R²) of the concentration spiked and concentration determined were 0.9411 in swine and 0.9623 in cattle. Therefore, this assay has the potential for the rapid screening of Rac residue in food. 

Keywords:

Ractopamine, artificial antigen, polyclonal antibody, indirect competitive ELISA, heterology

---

Journal: Journal of Food, Agriculture and Environment
Year: 2012
Volume: 10
Issue: 2
Category: Environment
Pages: 1349-1352

---

Full text for Subscribers

---

Information:

Note to users

The requested document is freely available only to subscribers/registered users with an online subscription to the Journal of Food, Agriculture & Environment. If you have set up a personal subscription to this title please enter your user name and password. All abstracts are available for free.

Article purchasing

If you like to purchase this specific document such as article, review or this journal issue, contact us. Specify the title of the article or review, issue, number, volume and date of the publication. Software and compilation, Science & Technology, all rights reserved. Your use of this website details or service is governed by terms of use. Authors are invited to check from time to time news or information.

---

Purchase this Article:   20 Purchase PDF Order Reprints for 15

---

Share this article :