Journal of Food, Agriculture and Environment
Vol 8,
Issue 3&4,2010
Online ISSN: 1459-0263
Print ISSN: 1459-0255
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Distribution of Infectious Pancreatic Necrosis Virus (IPNV) in two major rainbow trout fry producing provinces of Iran with respect to clinically infected farms
Author(s):
Mehdi Raissy 1*,
Hassan Momtaz 1, Mahsa Ansari 2, Manouchehr Moumeni 3, Mehdi Hosseinifard 4Recieved Date: 2010-07-29, Accepted Date: 2010-11-12
Abstract:Reverse transcriptase polymerase chain reaction (RT-PCR) was applied to the detection and studying of distribution of infectious pancreatic necrosis virus (IPNV) using primer pairs designed for the amplification of vp2 gene fragment of the virus. The product of 206 bp was amplified from the viral RNA extracts of the tissues of suspected fish. A total number of 190 clinically suspected rainbow trout fry from 38 hatchery farms in Chaharmahal va Bakhtyari and Kohkilouye va Boyerahmad Provinces, both in west Iran, were studied and fishes of 12 hatchery farms (31.5% of all studied farms) found to be infected with IPNV. The results also showed that 8 and 4 hatchery farms found to be IPN-positive in Chaharmahal va Bakhtyari and Kohkilouye va Boyerahmad Provinces, respectively. The RT-PCR amplification of IPN vp2 is also recommended as a simple, highly specific and sensitive method allowing diagnosis of IPN within a short time.
Keywords:Reverse transcriptase polymerase chain reaction,
Infectious Pancreatic Necrosis, rainbow trout, IranJournal: Journal of Food, Agriculture and Environment
Year: 2010
Volume: 8
Issue: 3&4
Category: Agriculture
Pages: 614-615
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