Journal of Food, Agriculture and Environment

Vol 10, Issue 3&4,2012
Online ISSN: 1459-0263
Print ISSN: 1459-0255

Survivability of free and microencapsulated Lactobacillus plantarum with alginate and resistant starch in simulated gastrointestinal conditions


Yahya Shafiei 1*, Vadood Razavilar 2, Afshin Javadi 3, Hamid Mirzaei 3

Recieved Date: 2012-06-02, Accepted Date: 2012-09-18


The main objective of this study was to examine survivability of free and microencapsulated Lactobacillus plantarum PTCC 1058 with alginate and resistant starch in simulated gastrointestinal conditions. Mixtures of sodium alginate (2% w/v) and resistant starch (2% w/v) were prepared and pure bacterial mass (1% v/v) was added into the mixture. Then microcapsules were prepared by the use of emulsion method. Mean size of beads was 19.87 ±1.49 µm obtained by the Scanning Electron Microscopy (SEM). The survivability of free and encapsulated cells of L. plantarum was examined in 0.6% (w/v) bile salt solution (pH 8.25), simulated gastric juice (pH 1.55) and simulated intestinal juice with and without 0.6% (w/v) bile salt (pH 7.43) by incubation at 37oC with 50 rpm mechanical tension for 120 min. Survivability was investigated every 30 min by pour plate culture in MRS agar and incubation at 37oC for 72 h. All of the stages were carried out with triplicate trials. Encapsulated L. plantarum could reach to the colon by the viable count of 6.48 and 7.23 log CFU/ml live cells, respectively, at the presence and absence of bile salts in the intestinal juice, whereas, free bacteria could reach to the colon by 4.6 log CFU/ml live cells which is not sufficient for its probiotic function. Statistical analyses with ANOVA and independent t-test showed that the survivability of encapsulated bacteria was significantly higher than that of free bacteria (P<0.05). It is concluded that microencapsulation of L. plantarum with calcium alginate and resistant starch can effectively protect these bacteria against gastrointestinal adverse conditions.


Lactobacillus plantarum, microencapsulation, simulated gastrointestinal conditions, survivability, alginate, resistant starch

Journal: Journal of Food, Agriculture and Environment
Year: 2012
Volume: 10
Issue: 3&4
Category: Food and Health
Pages: 207-212

Full text for Subscribers

Note to users

The requested document is freely available only to subscribers/registered users with an online subscription to the Journal of Food, Agriculture & Environment. If you have set up a personal subscription to this title please enter your user name and password. All abstracts are available for free.

Article purchasing

If you like to purchase this specific document such as article, review or this journal issue, contact us. Specify the title of the article or review, issue, number, volume and date of the publication. Software and compilation, Science & Technology, all rights reserved. Your use of this website details or service is governed by terms of use. Authors are invited to check from time to time news or information.

Purchase this Article:   20 Purchase PDF Order Reprints for 15

Share this article :