Journal of Food, Agriculture and Environment

Vol 10, Issue 3&4,2012
Online ISSN: 1459-0263
Print ISSN: 1459-0255

Multiplex PCR detection of Salmonella spp., Listeria monocytogenesEscherichia coli O157:H7, and Staphylococcus aureus in processed foods using [TA10] simultaneous growth broth


Susumu Kawasaki 1*, Kaori Kusano 2, Ritsuko Arai 2, Toshihiro Komeda 2, Naoko Kamisaki-Horikoshi 3, Shinichi Kawamoto 1

Recieved Date: 2012-07-15, Accepted Date: 2012-09-24


A multiplex (m-)PCR method was developed for simultaneous detection of Salmonella spp., Listeria monocytogenes, Escherichia coli O157:H7 and Staphylococcus aureus in processed foods. The m-PCR detection protocol consisted of three steps: 1) 20-h pre-enrichment culturing, 2) simple DNA extraction, and 3) m-PCR detection. For pre-enrichment of pathogens from food samples, [TA10] simultaneous growth broth was used.  [TA10] broth does not contain selective agents and is of practical use for these four pathogenic bacteria without modification. For m-PCR detection, the targeted genes included a Salmonella species-specific sequence within a 1.8-kb HindIII DNA fragment, L. monocytogenes hlyA, E. coli O157:H7 eaeA, and S. aureus femA. The m-PCR assay with enrichment broth was evaluated in 13 types of spiked food samples (including pasteurized milk, yogurt, processed cheese, etc.), and targeted genes specific for each pathogen simultaneously. Assay sensitivity was 10° CFU for each pathogen in 25 g of sample with a 20-h enrichment period. This m-PCR method is reliable and useful for rapid screening of processed food samples contaminated with Salmonella spp., L. monocytogenes, E. coli O157:H7 or S. aureus


Multiplex PCR, detection, Salmonella spp., Listeria monocytogenes, Escherichia coli O157:H7, Staphylococcus aureus

Journal: Journal of Food, Agriculture and Environment
Year: 2012
Volume: 10
Issue: 3&4
Category: Food and Health
Pages: 261-266

Full text for Subscribers

Note to users

The requested document is freely available only to subscribers/registered users with an online subscription to the Journal of Food, Agriculture & Environment. If you have set up a personal subscription to this title please enter your user name and password. All abstracts are available for free.

Article purchasing

If you like to purchase this specific document such as article, review or this journal issue, contact us. Specify the title of the article or review, issue, number, volume and date of the publication. Software and compilation, Science & Technology, all rights reserved. Your use of this website details or service is governed by terms of use. Authors are invited to check from time to time news or information.

Purchase this Article:   20 Purchase PDF Order Reprints for 15

Share this article :