Journal of Food, Agriculture and Environment

Agronomic characteristics of transgenic tobacco doubled haploids resistant to Potato virus Y

Author(s):

Anna Czubacka *, Teresa Doroszewska, Anna Trojak-Goluch

Recieved Date: 2012-06-20, Accepted Date: 2012-09-29

Abstract:

Transgenesis allows to modify plant genomes relatively quickly and precisely. Moreover, comparing with classical methods genetic transformation significantly facilitates and shortens the time needed for breeding a new cultivar. Shortening a breeding process is possible thanks to obtaining haploids and then doubled haploids being homozygotes as regards all traits which increase the efficiency of selection of desired recombinants. The plant material used in the study were transgenic tobacco doubled haploids containing Lettuce mosaic virus coat protein gene (LMV CP), which determines resistance to Potato virus Y (PVY). The doubled haploids (DH) were previously obtained from hybrids coming from crossing transgenic cultivar MN 944 with cv. Wiślica. DH plants were obtained by combination of anther culture and spontaneous doubling number of chromosomes under in vitro conditions. In the present study, the generation DH₂ of doubled haploid lines obtained by self-pollination were estimated by comparison with their parental cultivars. The experiment was conducted under greenhouse conditions. The evaluation of agronomic traits included elements of growth and development such as growth rate, number of days to flowering, area of leaves, height and morphology of plants. Cured leaves of DH lines were analyzed as regards selected physical and chemical traits. Individual DH lines differed among themselves and in comparison to parental cultivars as regards some traits. However, only little diversity within the lines was noticed. The DH lines characterized by the most satisfying agronomic traits may be valuable initial material in further breeding. 

Keywords:

DH, doubled haploid, LMV CP, Lettuce mosaic virus coat protein gene, PVY, Potato virus Y

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Journal: Journal of Food, Agriculture and Environment
Year: 2012
Volume: 10
Issue: 3&4
Category: Agriculture
Pages: 374-378

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