Journal of Food, Agriculture and Environment

Relationship of seasonal changes in antioxidative enzymes and cold-hardiness in strawberry plant

Author(s):

 Ece Turhan ¹*, Cigdem Aydogan ¹, Anil Akoglu ², Ali Baykul ², Yasemin Evrenosoglu ²

Recieved Date: 2012-07-02, Accepted Date: 2012-09-20

Abstract:

In this study, seasonal patterns of antioxidative enzymes and nicotinamide adenine dinucleotide phosphate (NADPH) oxidase (EC 1.6.3.1) activities and their relations to cold-hardiness of eight strawberry (Fragaria x ananassa) cultivars [Camarosa, Camino Real, Cal Giant 2 (CG 2), Cal Giant 4 (CG 4), Kabarla, Selva, Sweet Charlie and Ventana] were investigated. Fully expanded, uniformly sized leaves from about 1-year-old field plants of the cultivars were collected in cold-acclimated (CA, in January) and non-acclimated (NA, in July) stages. Leaf samples were exposed to low temperature at 5, -5, -10,  -20 and -30°C for 12 h to determine their cold-hardiness (LT₅₀; assessed by electrolyte leakage). A remarkable increase in cold-hardiness was observed with decreasing temperature. Ventana was found to have the highest cold-hardiness among the eight cultivars investigated. This cultivar has followed by Camino Real and CG 2 cultivars. CG 4 and Sweet Charlie have moderate cold-hardiness, while Selva and Camarosa were more sensitive than these two cultivars. On the other hand, Kabarla has the lowest cold-hardiness among the eight cultivars tested. Activities of catalase (CAT: EC 1.11.1.6), ascorbate peroxidase (APX: EC 1.11.1.11),  glutathion reductase (GR: EC 1.6.4.2) and NADPH oxidase were correlated to changes in cold-hardiness. Activities of these enzymes were higher in the CA stage than in the NA stage. Activities of APX and GR significantly varied depending on the cold-acclimation stage and the cold-hardiness level of the cultivars. This study indicates that, elevated antioxidative enzymes especially APX and GR, may be determinants of cold-hardiness in strawberry plant.

Keywords:

Strawberry, Fragaria x ananassa, cold-hardiness, oxidative stress, antioxidative enzymes

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Journal: Journal of Food, Agriculture and Environment
Year: 2012
Volume: 10
Issue: 3&4
Category: Agriculture
Pages: 445-450

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