Journal of Food, Agriculture and Environment

Vol 10, Issue 3&4,2012
Online ISSN: 1459-0263
Print ISSN: 1459-0255

Analysis of phenylalanine ammonia-lyase gene expression in callus cells of hawthorn (Crataegus aronia L.)


A. F. Al Qurraan 1, J. S. Sawwan 1, A. M. Al Abdallat 1, 2*

Recieved Date: 2012-06-06, Accepted Date: 2012-09-28


Crataegus aronia is a small shrub that belongs to the Rosaceae family and has been known for the medicinal activity of its extracts due to the high levels of flavonoids and phenolic compounds. In this work, the gene expression patterns of CaPAL gene were monitored in C. aronia callus cell cultures subjected to different stress conditions. The qRT-PCR results revealed that the CaPAL gene is induced in response to visible and ultraviolet light, high sucrose and high 2,4-D levels. CaPAL gene expression reached its highest level after 8 h of exposing callus cells to the UV-light. Addition of 100 mM sucrose to the media enhanced the expression of the CaPAL gene during the first 48 h of culturing when compared with 10 mM level cultured cells. Incubation of callus cells in continuous light increased the expression levels of CaPAL up to 48 h. High CaPAL expression levels were observed in callus cells cultured on MS media supplemented with 18 µM 2,4-D when compared with cells cultured on lower 2,4-D concentrations. These data reflect the effects of such treatments at the molecular level on the phenylpropanoid pathway in C. aronia callus culture. 


2,4-dichlorophenoxyacetic acid, light, phenylpropanoid pathway, sucrose, ultraviolet

Journal: Journal of Food, Agriculture and Environment
Year: 2012
Volume: 10
Issue: 3&4
Category: Agriculture
Pages: 572-576

Full text for Subscribers

Note to users

The requested document is freely available only to subscribers/registered users with an online subscription to the Journal of Food, Agriculture & Environment. If you have set up a personal subscription to this title please enter your user name and password. All abstracts are available for free.

Article purchasing

If you like to purchase this specific document such as article, review or this journal issue, contact us. Specify the title of the article or review, issue, number, volume and date of the publication. Software and compilation, Science & Technology, all rights reserved. Your use of this website details or service is governed by terms of use. Authors are invited to check from time to time news or information.

Purchase this Article:   20 Purchase PDF Order Reprints for 15

Share this article :