Journal of Food, Agriculture and Environment

Which housekeeping gene can be well used in gene expression analysis in Chinese local pig breeds?

Author(s):

Ze-Meng Feng ^{1, 3}, Jie-Ping Guo ^{1, 3}, Xiang-Feng Kong ¹ *, Yu-Long Yin ¹*, Guoqi Lian ^1,3, Dezhi Fu ^1,3, Hua Shao ^1,2, Charler Martin Nyachoti⁴

Recieved Date: 2012-06-30, Accepted Date: 2012-10-02

Abstract:

Relative quantification real-time PCR (RT-PCR) is widely used for the quantification of mRNA expression level. Before getting any conclusion, the expression results should be normalized by using one or more reference genes. In this study, three frequently-used endogenous reference genes, including glyceraldehyde-3-phosphate dehydrogenase (GAPDH), β-actin (ACTNB) and 18S rRNA (18S), were evaluated by comparing their expression levels in 864 samples from five Chinese local pig breeds (Huangjiang mini-pig, Bama mini-pig, Taoyuan black pig, Lantang pig and Ningxiang pig) and landrace pig (a foreign pig breed). Results showed that GAPDH and ACTNB had higher stable expression levels in all of the tissues determined in the present study, which indicated that both of them could be used as reference genes for the quantification of mRNA expression in some Chinese local pig breeds. ACTNB might be more suitable as reference gene for target genes with high expression level, while GAPDH might be more suitable to target genes with low expression level in studies on Chinese local pig breeds. Our findings also suggested that a prior evaluation of reference genes is necessary before researches were done in a new field, particularly for researches among different local pig breeds. Otherwise, absolute quantification RT-PCR should be recommend for these researches. 

Keywords:

Reference gene, real-time PCR, local pig breeds, mRNA expression

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Journal: Journal of Food, Agriculture and Environment
Year: 2012
Volume: 10
Issue: 3&4
Category: Agriculture
Pages: 825-833

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