Journal of Food, Agriculture and Environment

Vol 10, Issue 3&4,2012
Online ISSN: 1459-0263
Print ISSN: 1459-0255

Possible involvement of H2O2 induced Ca2+ efflux in the dormancy-breaking effect of hydrogen cyanamide and high temperature in nectarine floral buds


Yue Tan 1, Ling Li 1, Chen Shan Xu 2, Xiu De Chen 1, Dong Mei Li 1, Dong Sheng Gao 1

Recieved Date: 2012-06-08, Accepted Date: 2012-10-03


Dormancy is the main obstacle for the commercial production of temperate fruits in warm-winter regions and the protected fruit production now developing rapidly in China. Hydrogen cyanamide and high temperature are effective in the breakage of dormancy in nectarine and other deciduous trees, however, the details of mechanism are still unknown. Enhancements in the levels of H2O2 and elimination of high cytosolic Ca2+ are important processes in the dormancy release mechanism. Here, we reported that both hydrogen cyanamide and high temperature induced the increase of H2O2 in dormant nectarine floral buds. Exogenous application of H2O2 caused a shift of Ca2+ influx towards an efflux in dormant nectarine floral primordia. The observation indicated the possible involvement of H2O2 induced cytosolic Ca2+ elimination in dormancy release mechanism and provided new visions on H2O2/Ca2+ cooperation. Further experiments showed that the H2O2 induced Ca2+ flux shift could be inhibited by the pretreatment of LaCl3 (inhibitor of Ca2+-permeable channels) but not erythrosin B (inhibitor of Ca2+-ATPase), suggesting the key role of Ca2+-permeable channels in the process. In addition, H2O2 application also influenced the transportation of H+ and Mg2+, which was associated with cell cycle regulations and could also be involved in dormancy release mechanism. No significant transporting changes of Na+ and K+ were detected following the application of H2O2


Nectarine, floral buds, hydrogen peroxide, calcium, dormancy

Journal: Journal of Food, Agriculture and Environment
Year: 2012
Volume: 10
Issue: 3&4
Category: Agriculture
Pages: 846-849

Full text for Subscribers

Note to users

The requested document is freely available only to subscribers/registered users with an online subscription to the Journal of Food, Agriculture & Environment. If you have set up a personal subscription to this title please enter your user name and password. All abstracts are available for free.

Article purchasing

If you like to purchase this specific document such as article, review or this journal issue, contact us. Specify the title of the article or review, issue, number, volume and date of the publication. Software and compilation, Science & Technology, all rights reserved. Your use of this website details or service is governed by terms of use. Authors are invited to check from time to time news or information.

Purchase this Article:   20 Purchase PDF Order Reprints for 15

Share this article :