Journal of Food, Agriculture and Environment

In vitro chromosome doubling and tetraploid identification in Lagerstroemia indica 

Author(s):

Xiao-Jiao Wang ¹*, Xue-Feng Wang ^{1, 3}*, Ming Cai ¹, Dan He ¹, Hui-Tang Pan ^{1, 2}, Qi-Xiang Zhang ^{1, 2}*

Recieved Date: 2012-06-20, Accepted Date: 2012-09-28

Abstract:

We developed a sterile culture system suitable for in vitro chromosome doubling using cluster buds or shoot tips of Lagerstroemia indica ‘Bicolor’. Cluster buds and shoot tips were treated with various concentrations of colchicine solution (0.2%, 0.5%, 0.8%) and incubated for 6, 12 or 24 h on an orbital shaker at 100 rpm. They were then cultured on half Murashige and Skoog (MS) medium supplemented with 1.0 mg l^-1 6-benzyladenine (BA) and 0.1 mg l^-1 indole-3-butyric acid (IBA) for the induction of cluster buds, and subsequently transferred to half MS medium supplemented with 0.05 mg l^-1 1-naphthaleneacetic acid (NAA) for rooting. The highest frequency (36%) of mutated plants was obtained by immersing the shoot tips in 0.5% colchicine for 12 h. The ploidy level of putative tetraploid plants was verified by flow cytometry and chromosome counts. Tetraploid plantlets showed darker and rougher green leaves, larger stomata guard cells and lower density of stomata. 

Keywords:

Lagerstroemia indica, chromosome doubling, colchicine, stomata, tetraploid, flow cytometry

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Journal: Journal of Food, Agriculture and Environment
Year: 2012
Volume: 10
Issue: 3&4
Category: Environment
Pages: 1364-1367

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