Journal of Food, Agriculture and Environment
Vol 10,
Issue 3&4,2012
Online ISSN: 1459-0263
Print ISSN: 1459-0255
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Callus induction and plantlet regeneration from axillary bud of Triarrhena lutarioriparia var. lutarioriparia
Author(s):
Yan-yun Hong 1,
Ju-yun Hong 2, Hai-rui Duo3, Ting-ting Wei 1, Tu-yong Yi 4, 5*Recieved Date: 2012-07-21, Accepted Date: 2012-10-01
Abstract:To establish the regeneration system of axillary buds from Triarrhena lutarioriparia var. lutarioriparia, several induction media with different plant growth regulators concentrations were orthogonally designed to induce callus. The results showed that KT and 6-BA were the key factors to the induction and differentiation of callus of axillary buds, and calli were found to emerge after 7-10 d. The callus induction frequency (CIF) and callus differentiation frequency (CDF) were 42% and 85%.The highest number of seedlings per callus was regenerated after the third subculture, the lowest number of seedlings per callus after the fourth subculture.
Keywords:T. lutarioriparia var. lutarioriparia,
axillary bud, callus induction, plantlet regenerationJournal: Journal of Food, Agriculture and Environment
Year: 2012
Volume: 10
Issue: 3&4
Category: Environment
Pages: 1382-1385
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