Journal of Food, Agriculture and Environment

Isolation, identification and analysis of DDT-degrading bacteria for agriculture area improvements

Author(s):

Pannapa Powthong ¹*, Bajaree Jantrapanukorn ¹, Pattra Suntornthiticharoen ²

Recieved Date: 2015-09-10, Accepted Date: 2015-12-23

Abstract:

Dichlorodiphenyltrichloroethane (DDT) is an organochlorine pesticide that can persist in the environment resulting in environmental problem with chronic effects on human and animal health. Biodegradation is a potential method to detoxify the recalcitrant compounds. In this study, the potential for biodegradation of DDT through enrichment and isolation of DDT-degrading bacteria from soil and water in agricultural area was done. Microorganisms grew in minimal media with DDT as the only carbon source. Six from 40 bacterial isolates were selected by increasing concentrations of DDT. The DDT degradation was quantified by the enrichment cultures degrading S4-67.95%, S13-65.05%, S37-65.45%, S39-67.25%, W30-65.20% and W37-67.55% of the initial amount of DDT, respectively, in 7 days. Further, the optimal of temperature, pH, and salinity of the isolate has also been investigated and revealed that an optimum temperature at 37–45ºC was able to growth at a wide range of NaCl and pH. These promising DDT-degrading isolates were tentatively identified biochemical and MALDI-TOF MS characterization as 3 groups: Pseudomonas stutzeri (3 isolates), Pseudomonas aeruginosa (2 isolates) and Bacillus firmus (1 isolate).

Keywords:

DDT, biodegradation, pesticide, bioremediation, organochlorine compounds

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Journal: Journal of Food, Agriculture and Environment
Year: 2016
Volume: 14
Issue: 1
Category: Environment
Pages: 131-136

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