Journal of Food, Agriculture and Environment

Impact of solvent type on antibacterial activities of Lawsonia inermis leaves

Author(s):

Ibtisam Mohammed Ababutain

Recieved Date: 2014-09-02, Accepted Date: 2014-12-20

Abstract:

The objective of this study was to determine the best solvent that can be used to obtain higher antibacterial activity of Lawsonia inermis leaves against nine bacterial species, five Gram-negative bacteria Escherichia coli, Enterobacter aerogenes, Pseudomonas aeruginosa, Delftia tsuruhatensis and Serratia liquefaciens and four Gram-positive bacteria Streptococcus pyogenes, Staphylococcus aureus, Bacillus subtilis and Bacillus cereus. The results showed that the aqueous extract had the best inhibitory influence on 8 out of 9 test bacteria, followed by ethanol extract inhibiting 7 out of 9 test bacteria, while acetone extract was weakest inhibiting only 3 out of 9 test bacteria. Therefore, choosing the right solvent can be considered as an important step to obtain best results. This may indicate that some active substances in L. inermis dissolve in varying degrees in different solvents. Our results indicated that water followed by ethanol were the best solvents for the active substances lawsone, 2-hydroxy-1,4-naphthoquinone C₁₀H₆ O₃ which have antibacterial potential in L. inermis leaves.

Keywords:

Antibacterial activity, lawsone, henna extracts, solvent

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Journal: Journal of Food, Agriculture and Environment
Year: 2015
Volume: 13
Issue: 1
Category: Agriculture
Pages: 51-53

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