Journal of Food, Agriculture and Environment

Vol 11, Issue 1,2013
Online ISSN: 1459-0263
Print ISSN: 1459-0255

Selecting the optimal levels of non-starch polysaccharides (NSP) degrading enzymes for NSP degradation in selected feed ingredient 


Zikui Liu 1, Tiejun Li 2*, Fugui Yin 2*, Shengping Wang 2, Jie Wang 1, Zhichun Zhan 3, Ying Zhou 3, Ruilin Huang 2*

Recieved Date: 2012-10-20, Accepted Date: 2013-01-12


Two experiments were conducted to select the optimal levels of either a single or a combination of two or three non-starch polysaccharides (NSP) degrading enzymes for NSP degradation in a selected cottonseed meal (protein content of which is low, collected from Xinjiang, China). In Exp. 1, the cottonseed meal was incubated with different levels of NSP-degrading enzymes, including xylanase, mannanase, glucanase and pectinase, at 40°C for 2 h in quadruplicates to measure the in vitro digestibility of NSP fractions. In Exp. 2, nine ileal cannulated pigs with an average body weight of 16 (SD 1.41) kg were assigned to nine dietary treatments in a 9 × 9 Latin square design. Diets were prepared by adding either a single or a combination of NSP- degrading enzymes at the appropriate level determined in Exp.1. Diets were fed for five days followed by a 24 h ileal digesta collection period for determining the apparent ileal digestibility (AID) of dry matter, energy, crude protein and NSP fractions. Total NSP contents in the selected grains and other agricultural by-products were relatively low (less than 14%), except for that of barley (sample ID C-4); however, that in other agricultural by-products were relatively high, and some even up to 49.9% (for example palm kernel meal, sample ID T-4). The content of water-soluble non- starch polysaccharides (WSNSP) was lower than that of water-insoluble NSP in all the tested feed ingredients. The monosaccharide contents were variable among feed sources and origins. The optimal levels of NSP-degrading enzymes per kg of sample for in vitro degradation of water-soluble and total NSP in the selected cottonseed meal were: xylanase (1000 U/kg), mannase (6000 U/kg), glucanase (2000 U/kg) and pectinase (3000 U/kg), respectively. The AID of dry matter, gross energy, crude protein, total WSNSP, arabinose and galactose in pectinase-supplemented (3000 U/kg) diet were higher (P < 0.05) than in control and in diets supplemented with xylanase, mannanase or glucanase. Collectively, dietary supplementation with NSP-degrading enzymes could effectively degrade dietary WSNSP and total NSP and therefore improve the digestibility of main nutrients and NSP fractions, and such function of pectinase was even better than that of combination of NSP-degrading enzymes in pigs. 


Pigs, fiber, non-starch polysaccharides degrading enzyme, cottonseed meal, digestibility

Journal: Journal of Food, Agriculture and Environment
Year: 2013
Volume: 11
Issue: 1
Category: Agriculture
Pages: 428-435

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